CAJ | 학술논문

以紫色视杆菌Chromobacterium violaceum 026为报告菌株,以己酰基高丝氨酸内酯作为菌株生长的唯一碳源及能源,通过富集培养、分离纯化,从西南印度洋海泥中筛选得到2株菌S2和S3。检测结果表明,S2、S3可能具有胞内AHL降解酶活性。形态鉴定和16S rDNA序列分析表明,2株菌均为芽孢杆菌属。系统发育分析表明,菌株S2、S3与Bacillus amyloliquefaciens的亲缘关系最近。
이자색시간균Chromobacterium violaceum 026위보고균주,이기선기고사안산내지작위균주생장적유일탄원급능원,통과부집배양、분리순화,종서남인도양해니중사선득도2주균S2화S3。검측결과표명,S2、S3가능구유포내AHL강해매활성。형태감정화16S rDNA서렬분석표명,2주균균위아포간균속。계통발육분석표명,균주S2、S3여Bacillus amyloliquefaciens적친연관계최근。
With N-hexanoyl homoserine lactone as the sole carbon and erengy source for growth of microorganisms and Chromobacterium violaceum 026 as an indicator bacteria, two strains S2 and S3 were screened from southwest Indian sea mud by enrichment, isolation and purification. Results showed that S2 and S3 possibly had intracellular AHL degradation enzymes. The two strains were identified as Bacillus genus by morphological identification and 16 S rDNA sequence analysis. Phylogenetic analysis demonstrated that S2 and S3 were most relative to Bacillus amyloliquefaciens.