CAJ | 학술논문

采用高效液相色谱法HPLC对微透析液进行痕量脯氨酸的检测,研究了痕量脯氨酸柱前衍生化的方法。采用苯异硫氰酸酯PITC-乙腈溶液和三乙胺-乙腈溶液对透析液中痕量脯氨酸进行衍生化后,通过环己烷萃取,得到透析液中痕量脯氨酸衍生化的最适方法,无需后续处理可直接进样,并确定了HPLC检测条件：岛津Inertsil ODS-SP色谱柱(250 mm ×4.6 mm,5μm)；柱温：31℃；流动相A：0.05 mol·L-1醋酸钠溶液；流动相 B：乙腈-流动相 A(1：1)；洗脱条件：A：B(50：50)；流速：1.0 mL· min-1；检测波长：254 nm；进样量：20μL。脯氨酸加标回收率为95.1%~104.2%。同时,对3种杨树质外体透析液中的脯氨酸进行了测量。
채용고효액상색보법HPLC대미투석액진행흔량포안산적검측,연구료흔량포안산주전연생화적방법。채용분이류청산지PITC-을정용액화삼을알-을정용액대투석액중흔량포안산진행연생화후,통과배기완췌취,득도투석액중흔량포안산연생화적최괄방법,무수후속처리가직접진양,병학정료HPLC검측조건：도진Inertsil ODS-SP색보주(250 mm ×4.6 mm,5μm)；주온：31℃；류동상A：0.05 mol·L-1작산납용액；류동상 B：을정-류동상 A(1：1)；세탈조건：A：B(50：50)；류속：1.0 mL· min-1；검측파장：254 nm；진양량：20μL。포안산가표회수솔위95.1%~104.2%。동시,대3충양수질외체투석액중적포안산진행료측량。
Based on microdialysis experience in apoplast of poplar stems, the trace of proline in plant was detected by using the High -performance liquid chromatography ( HPLC ) method, precolumn derivatization method was studied. Phenyl isothiocyanate PITC- acetonitrile solution and triethylamine acetonitrile solution were applied to derivate the proline. Cyclohexane was used to extract proline, the sample could be directly injected in HPLC without subsequent pro-cessing. The detection conditions were determined: the SHIMADZU Inertsil ODS-SP column (250 mm × 4. 6 mm, 5 μm);column temperature:31 ℃;mobile phase A:0. 05mol·L-1 so-dium acetate solution, mobile phase B: acetonitrile-mobile phase A (1:1); Elution condi-tions:A:B (50:50);flow rate:1. 0 mL·min-1;detection wavelength:254nm;the injection volume was 20μL. The recovery rate of proline was 95. 1 -104. 2%. At last the proline con-tents of three poplar apoplast dialysis were detected by this method.