CAJ | 학술논문

采用聚丙烯酰胺凝胶电泳技术，分析了兰州鲇（Silurus lanzhouensis）肌肉、心脏、肝脏、脑、脾脏、肾脏和眼睛等7种组织中乳酸脱氢酶（LDH）、酯酶（EST）、苹果酸脱氢酶（MDH）、醇脱氢酶（ADH）、山梨醇脱氢酶（SDH）和谷氨酸脱氢酶（GDH）等6种同工酶的表达模式，并对各同工酶的酶谱进行了分析。结果表明：这6种同工酶在兰州鲇7种组织中的分布均存在明显的组织特异性；6种酶共记录出17个基因位点，其中 EST－2、 EST－3、 MDH－3和 SDH－2为多态位点；兰州鲇群体的多态位点比例 P 为23．53％，位点平均有效等位基因数 Ne 为1．24；平均观测杂合度和平均期望杂合度分别为0．1372和0．1051， Hardy－Weinberg 遗传偏离指数D 为0．3054，杂合子处于过剩状态。从以上数据中可以看出，兰州鲇野生群体遗传变异程度较大，但是有效等位基因数偏低，有必要对兰州鲇野生种质资源及时加以保护。
채용취병희선알응효전영기술，분석료란주점（Silurus lanzhouensis）기육、심장、간장、뇌、비장、신장화안정등7충조직중유산탈경매（LDH）、지매（EST）、평과산탈경매（MDH）、순탈경매（ADH）、산리순탈경매（SDH）화곡안산탈경매（GDH）등6충동공매적표체모식，병대각동공매적매보진행료분석。결과표명：저6충동공매재란주점7충조직중적분포균존재명현적조직특이성；6충매공기록출17개기인위점，기중 EST－2、 EST－3、 MDH－3화 SDH－2위다태위점；란주점군체적다태위점비례 P 위23．53％，위점평균유효등위기인수 Ne 위1．24；평균관측잡합도화평균기망잡합도분별위0．1372화0．1051， Hardy－Weinberg 유전편리지수D 위0．3054，잡합자처우과잉상태。종이상수거중가이간출，란주점야생군체유전변이정도교대，단시유효등위기인수편저，유필요대란주점야생충질자원급시가이보호。
Polyacrylamide gel electrophoresis was used to investigate the expression pattern and isozyme zymogram of six isozymes (LDH, EST, MDH, ADH, SDH and GDH) in 7 tissues (muscle, heart, liver, brain, spleen, kidney and eye) of Silurus lanzhouensis.The results showed that: the expression of all the isozymes studied were highly tissue -specific in S.lanzhouensis; 17 enzyme-coding loci were examined, of which four loci were polymorphic , i.e., EST-2, EST-3, MDH-3 and SDH-2; the proportion of polymorphic loci was 23.53%, while the average effective number of alleles (Ne) was1.24; the average values of observed and expected heterozygosity were 0.137 2 and 0.105 1, respectively; the Har-dy-Weinberg deviation index was 0.305 4, which showed that heterozygotes were surplus .The above data indicated that the genetic variation ofS.lan zhouensis wild population was relatively high ; however, the average effective number of alleles was quite low.The protection of wild resources of S.lanzhouensis should be conducted in time.