CAJ | 학술논문

巨（Bagarius yarrelli）为云南特有的经济鱼类，利用随机扩增多态性 DNA（RAPD）技术对元江下游的河口巨野生群体进行了遗传多样性分析，在50条随机引物中筛选出19条多态性较好的引物，通过 PCR 扩增，19条引物在巨群体中共检测到67个位点，其中多态性位点66个，平均多态性位点比率为98．51％，个体间平均遗传相似系数为0．8909，个体间平均遗传距离为0．1176；群体 Nei′s 基因多样性指数为0．2916， Shannon 信息指数为0．4269，结果表明巨野生群体具有较高的遗传多样性。
거（Bagarius yarrelli）위운남특유적경제어류，이용수궤확증다태성 DNA（RAPD）기술대원강하유적하구거야생군체진행료유전다양성분석，재50조수궤인물중사선출19조다태성교호적인물，통과 PCR 확증，19조인물재거군체중공검측도67개위점，기중다태성위점66개，평균다태성위점비솔위98．51％，개체간평균유전상사계수위0．8909，개체간평균유전거리위0．1176；군체 Nei′s 기인다양성지수위0．2916， Shannon 신식지수위0．4269，결과표명거야생군체구유교고적유전다양성。
Bagarius yarrelli is an ecomomic endemic fish in the Red River , in Yunnan Province, China.The genetic di-versity of B.yarrelli populations from Hekou was studied by random amplified polymorphic DNA (RAPD) technology.19 polymorphic primers were screened in 50 random primers by PCR(Polymerase Chain Reaction), 67 loci were detected in B.yarrelli fish groups, of which 66 loci were polymorphic loci, the average ratio was 98.51%, The average genetic simi-larity index and average genetic distance were 0.890 9 and 0.117 6, respectively; The Nei′s gene diversity index was 0.291 6 and shannon′s information index was 0.426 9.The results showed that there was a high genetic diversity of the Hekou B.yarrelli.The wild B.yarrelli population genetic diversity through RAPD analysis can provide reference for the ra -tional development and protection of B.yarrelli fish germplasm resources in the future.