淡水渔业
淡水漁業
담수어업
FRESHWATER FISHERIES
2015年
1期
73-78
,共6页
凌空%王涵%丁诗华%吴兴镇
凌空%王涵%丁詩華%吳興鎮
릉공%왕함%정시화%오흥진
豚鼠气单胞菌(Aeromonas caviae)%大鲵(Andrias davidianus)%中草药%抑菌作用
豚鼠氣單胞菌(Aeromonas caviae)%大鯢(Andrias davidianus)%中草藥%抑菌作用
돈서기단포균(Aeromonas caviae)%대예(Andrias davidianus)%중초약%억균작용
Aeromonas caviae%Andrias davidianus%Chinese herbal medicine%antibacterial activity
应用二倍稀释法测定了诃子、五倍子、石榴皮等16种中草药及其复方对大鲵（Andrias davidianus）致病性豚鼠气单胞菌（Aeromonas caviae）的最小抑菌浓度和最小杀菌浓度，应用棋盘法设计16种中草药组合的120种双联复方和10种中草药组合的120种三联复方，评估了复方的体外抑菌作用。结果表明：16种中草药对大鲵致病性豚鼠气单胞菌均有一定程度的抑菌作用，抑菌作用强弱依次为诃子、五倍子、石榴皮、大黄、槐角、覆盆子、黄芩、金樱子、肉苁蓉、丁香、川牛膝、赤芍、知母、防风、天葵子及罗汉果；在120个中草药双联复方中，5．8％为显著协同作用，82．6％为协同作用，11．6％为相加作用；在120个中草药三联复方中，29．2％为显著协同作用，87．5％为协同作用。因此，合理的中草药配伍能提高对大鲵致病性豚鼠气单胞菌的体外抑菌作用。
應用二倍稀釋法測定瞭訶子、五倍子、石榴皮等16種中草藥及其複方對大鯢（Andrias davidianus）緻病性豚鼠氣單胞菌（Aeromonas caviae）的最小抑菌濃度和最小殺菌濃度，應用棋盤法設計16種中草藥組閤的120種雙聯複方和10種中草藥組閤的120種三聯複方，評估瞭複方的體外抑菌作用。結果錶明：16種中草藥對大鯢緻病性豚鼠氣單胞菌均有一定程度的抑菌作用，抑菌作用彊弱依次為訶子、五倍子、石榴皮、大黃、槐角、覆盆子、黃芩、金櫻子、肉蓯蓉、丁香、川牛膝、赤芍、知母、防風、天葵子及囉漢果；在120箇中草藥雙聯複方中，5．8％為顯著協同作用，82．6％為協同作用，11．6％為相加作用；在120箇中草藥三聯複方中，29．2％為顯著協同作用，87．5％為協同作用。因此，閤理的中草藥配伍能提高對大鯢緻病性豚鼠氣單胞菌的體外抑菌作用。
응용이배희석법측정료가자、오배자、석류피등16충중초약급기복방대대예（Andrias davidianus）치병성돈서기단포균（Aeromonas caviae）적최소억균농도화최소살균농도，응용기반법설계16충중초약조합적120충쌍련복방화10충중초약조합적120충삼련복방，평고료복방적체외억균작용。결과표명：16충중초약대대예치병성돈서기단포균균유일정정도적억균작용，억균작용강약의차위가자、오배자、석류피、대황、괴각、복분자、황금、금앵자、육종용、정향、천우슬、적작、지모、방풍、천규자급라한과；재120개중초약쌍련복방중，5．8％위현저협동작용，82．6％위협동작용，11．6％위상가작용；재120개중초약삼련복방중，29．2％위현저협동작용，87．5％위협동작용。인차，합리적중초약배오능제고대대예치병성돈서기단포균적체외억균작용。
To study the inhibitory effects of sixteen kinds of Chinese herbal medicine and their compounds on pathogenic Aeromonas caviae from cultured Andrias davidianus in vitro, the minimum inhibitory concentration (MIC) and the mini-mum bactericidal concentration (MBC) of single drug and the compounds on pathogenic A.caviae from A.davidianus were measured with agar double dilution method.Then sixteen kinds of chinese herbal medicine were mixed one by one based on chessboard experimental method to get one hundred and twenty kinds of compounds of Chinese herbal medicine .The results showed that sixteen kinds of Chinese herbal medicine had antibacterial effect at certain extent on the pathogenic bacteri -a.Terminalia chebula Retz and Rhus chinensis Mil had the best antibacterial activity, followed by Pomegranate Rind, Rheum palmatum L, Sophora japonica L, Rubus idaeus, Scutellaria baicalensis, Rosa laevigata, Cistanche deserticola and Syringa Linn, Radix Cyathulae, Paeonia veitchii, Anemarrhena asphodeloides, DivaricateSaposhnikovia Root, Musk-root Semiaquilegia Root and Momordica grosvenori had the worst antibacterial activity.The study indicated that the rational use of Chinese herbal medicine could increase the inhibitory effects of the drug on A.caviae from A.davidianus in vitro drug Antibacterial activity.
