CAJ | 학술논문

运用电镜和免疫荧光技术，对纯化的大鲵（Andrias davidianus）虹彩病毒粒子、感染病毒的 EPC 细胞以及确诊感染病毒的病鲵组织样品进行观察和分析。结果表明，纯化的大鲵虹彩病毒负染后电镜下显示球形结构，具囊膜，直径约150 nm；感染 EPC 细胞中的病毒颗粒呈典型的正二十面体结构，由核衣壳和核心构成，核衣壳呈正六边形，对角直径为（150±5） nm （N ＝30），核衣壳厚度约5 nm，核心直径为（98±18） nm （N ＝27）。在病鲵病变的肺和肾组织中发现存在大量聚集或分散的病毒颗粒，其形态特征和感染 EPC 细胞超薄切片观察的结果一致。免疫荧光电镜观察结果显示，感染病毒的细胞可观察到明显的红色荧光信号，且呈斑块状分布，大小不等。综合大鲵虹彩病毒初步的形态发生和免疫荧光观察结果，认为病毒感染细胞后在不同的发生时期会形成不同类型的病毒包涵体。
운용전경화면역형광기술，대순화적대예（Andrias davidianus）홍채병독입자、감염병독적 EPC 세포이급학진감염병독적병예조직양품진행관찰화분석。결과표명，순화적대예홍채병독부염후전경하현시구형결구，구낭막，직경약150 nm；감염 EPC 세포중적병독과립정전형적정이십면체결구，유핵의각화핵심구성，핵의각정정륙변형，대각직경위（150±5） nm （N ＝30），핵의각후도약5 nm，핵심직경위（98±18） nm （N ＝27）。재병예병변적폐화신조직중발현존재대량취집혹분산적병독과립，기형태특정화감염 EPC 세포초박절편관찰적결과일치。면역형광전경관찰결과현시，감염병독적세포가관찰도명현적홍색형광신호，차정반괴상분포，대소불등。종합대예홍채병독초보적형태발생화면역형광관찰결과，인위병독감염세포후재불동적발생시기회형성불동류형적병독포함체。
Electron microscope and immunofluorescence microscope were used to observe and study the purified Chinese giant salamanders iridovirus (CGSIV) viral particles and infected EPC cells with CGSIV and visceral tissue samples of sick Chinese giant salamanders.The negatively stained electron micrographs revealed that CGSIV was ball -shape with envelops and about 150 nm in diameter.The electron micrographs of ultra -thin section of infected cells suggested that CGSIV virions showed typically morphological character of iridovirus , which possessed icosahedral symmetry with member envelopes , and CGSIV was composed of the capsid and nucleoid .Its capsid was regular hexagon in shape and (150 ±5) nm(N =30) in diameter, and the thickness of capsid was about 5 nm and the nucleoid was (98 ±18) nm (N =27) in diameter.CGSIV virions were found in lung and kidney of sick Chinese giant salamanders and possessed the similar ultrastructure as that ob -served in electron micrographs of ultra -thin section of infected cells.A large number of red fluorescent signals were detec -ted in CGSIV -infected cells by immunofluorescence assay , which were distributed as patches in cytoplasm .Based on the observation of ultra -thin section of infected EPC cells and fluorescence microscopy images , it was believed that different types of CGSIV inclusion bodies could be found in cytoplasm of the infected cells .