临床普外科电子杂志
臨床普外科電子雜誌
림상보외과전자잡지
Journal of General Surgery for Clinicians (Electronic Version)
2014年
2期
15-22
,共8页
张大伟%邢雪%邓乃梅%谭雪莹%汤海涛
張大偉%邢雪%鄧迺梅%譚雪瑩%湯海濤
장대위%형설%산내매%담설형%탕해도
肝细胞癌%血管紧张素Ⅱ%碱性成纤维细胞因子%细胞增殖
肝細胞癌%血管緊張素Ⅱ%堿性成纖維細胞因子%細胞增殖
간세포암%혈관긴장소Ⅱ%감성성섬유세포인자%세포증식
Hepatocellular Carcinoma%Angiotensin II%Basic Fibroblast Growth Factor%Cell Proliferation
目的：研究血管紧张素Ⅱ(AngII)对人肝癌HepG2细胞系中碱性成纤维生长因子(bFGF) mRNA表达的影响。方法体外培养HepG2肝癌细胞，以不同浓度的AngII对细胞进行药物处理并收集处理后不同时点的细胞，每组设3个对照组，采用酶联免疫吸附试验(ELISA)及荧光定量-聚合酶链反应( RT-PCR)法，分别检测AngII处理前后HepG2细胞中bFGF的表达，同时应用CCK-8法检测AngII对HepG2细胞增殖的影响。结果 AngII在刺激HepG2细胞增殖同时还上调细胞中bFGF mRNA的表达(P<0.01)，其作用随时间及浓度的增加而增强，当AngII浓度为10~7mol/L，时间为48小时，这种作用达到最高值，且此作用可被AngII1型受体(AT1R)拮抗剂所阻断。结论AngII通过诱导人肝癌细胞中bFGFmRNA的表达，上调肝癌细胞中bFGF的含量，从而一定程度上促进了肝癌的生长及转移。
目的：研究血管緊張素Ⅱ(AngII)對人肝癌HepG2細胞繫中堿性成纖維生長因子(bFGF) mRNA錶達的影響。方法體外培養HepG2肝癌細胞，以不同濃度的AngII對細胞進行藥物處理併收集處理後不同時點的細胞，每組設3箇對照組，採用酶聯免疫吸附試驗(ELISA)及熒光定量-聚閤酶鏈反應( RT-PCR)法，分彆檢測AngII處理前後HepG2細胞中bFGF的錶達，同時應用CCK-8法檢測AngII對HepG2細胞增殖的影響。結果 AngII在刺激HepG2細胞增殖同時還上調細胞中bFGF mRNA的錶達(P<0.01)，其作用隨時間及濃度的增加而增彊，噹AngII濃度為10~7mol/L，時間為48小時，這種作用達到最高值，且此作用可被AngII1型受體(AT1R)拮抗劑所阻斷。結論AngII通過誘導人肝癌細胞中bFGFmRNA的錶達，上調肝癌細胞中bFGF的含量，從而一定程度上促進瞭肝癌的生長及轉移。
목적：연구혈관긴장소Ⅱ(AngII)대인간암HepG2세포계중감성성섬유생장인자(bFGF) mRNA표체적영향。방법체외배양HepG2간암세포，이불동농도적AngII대세포진행약물처리병수집처리후불동시점적세포，매조설3개대조조，채용매련면역흡부시험(ELISA)급형광정량-취합매련반응( RT-PCR)법，분별검측AngII처리전후HepG2세포중bFGF적표체，동시응용CCK-8법검측AngII대HepG2세포증식적영향。결과 AngII재자격HepG2세포증식동시환상조세포중bFGF mRNA적표체(P<0.01)，기작용수시간급농도적증가이증강，당AngII농도위10~7mol/L，시간위48소시，저충작용체도최고치，차차작용가피AngII1형수체(AT1R)길항제소조단。결론AngII통과유도인간암세포중bFGFmRNA적표체，상조간암세포중bFGF적함량，종이일정정도상촉진료간암적생장급전이。
Objective To investigate the effects of angiotensinⅡ (Ang II) on the expression of basic fibroblast growth factor (bFGF) in human hepatic cancer cell line HepG2.Methods Cultured HepG2 were treated by Ang II with various concentrations and collected at different time points within which contained 3 control groups, introducing Enzyme-Linked immunosorbent assay (ELISA) and real time polymerase chain reaction(PCR) method to detect the expression of bFGF in HepG2 cell lines respectively, meantime apply cck-8 method to detect the influence of Ang II to HepG2 cells.Results Ang II stimulated the proliferation of HepG2 cells by increased bFGF protein expression and enhanced the expression of bFGF mRNA (P<0. 01) ,which was concentration and time dependent . The effect reach its maximum value that the concentration of Ang II reaches 10-7mol/L and the time reaches 48 hours and which could be suppressed by Angiotensin II type 1 receptor (AT1R) antagonist.Conclusion: The expression of bFGFmRNA can be induced by Ang II in human hepatic cancer cell line HepG2 through AT1R, which may play a deifnite role in tumor growth and metastasis.
