仔猪雷极氏普罗菲登斯菌的分离鉴定及系统进化分析
자저뢰겁씨보라비등사균적분리감정급계통진화분석
Isolation Identification and Phylogenetic Analysis of Providencia Rettgeri in Piglets
  • 万方数据
    动物医学进展 동물의학진전
    PROGRESS IN VETERINARY MEDICINE
    2015年 2期 43-48 ,共6页

    秦毅斌%何颖%赵武%卢冰霞%李斌%李莹莹%梁家幸%陈忠伟%梁保忠%苏乾莲%周英宁%蒋冬福%黎珂钰%卢敬专

    진의빈%하영%조무%로빙하%리빈%리형형%량가행%진충위%량보충%소건련%주영저%장동복%려가옥%로경전

    雷极氏普罗菲登斯菌%分离鉴定%16 S rRNA%系统进化 雷極氏普囉菲登斯菌%分離鑒定%16 S rRNA%繫統進化
    뢰겁씨보라비등사균%분리감정%16 S rRNA%계통진화
    Providencia rettgeri%isolation and identification%16 S rRNA%phylogenetic analysis
    为了解猪雷极氏普罗菲登斯菌（P ．rettgeri）的生物学特性、致病性及16 S rRNA基因系统进化关系，本研究从发生严重腹泻、血便的哺乳仔猪群的内脏器官中分离到1株病原菌，根据形态特征、培养特性、生化特性、16 S rRNA基因序列分析鉴定为 P ．rettgeri。小鼠攻毒试验证实该分离菌株对试验小鼠有较强致病力，哺乳仔猪回归试验可复制出与临床上相似的典型症状，并从发病死亡小鼠及哺乳仔猪中分离到攻毒菌株。系统进化分析表明，分离菌株与雷极氏普罗菲登斯菌系统进化关系最为密切，其16 S rRNA 基因序列与雷极氏普罗菲登斯菌代表菌株的同源性在97．3％～99．6％之间。药敏试验结果表明，分离菌株对头孢哌酮钠、头孢噻肟钠、头孢曲松钠、头孢唑啉、头孢呋肟、头孢吡肟、阿莫西林、链霉素、氨曲南、诺氟沙星、左氟沙星、恩诺沙星、卡那霉素、氨苄青霉素、阿米卡星、链霉素和阿米卡星等多数药物敏感。本研究首次报道了雷极氏普罗菲登斯菌可以引起哺乳仔猪严重腹泻，提示在仔猪腹泻中应注意该菌感染的诊断、监测和防控。
    위료해저뢰겁씨보라비등사균（P ．rettgeri）적생물학특성、치병성급16 S rRNA기인계통진화관계，본연구종발생엄중복사、혈편적포유자저군적내장기관중분리도1주병원균，근거형태특정、배양특성、생화특성、16 S rRNA기인서렬분석감정위 P ．rettgeri。소서공독시험증실해분리균주대시험소서유교강치병력，포유자저회귀시험가복제출여림상상상사적전형증상，병종발병사망소서급포유자저중분리도공독균주。계통진화분석표명，분리균주여뢰겁씨보라비등사균계통진화관계최위밀절，기16 S rRNA 기인서렬여뢰겁씨보라비등사균대표균주적동원성재97．3％～99．6％지간。약민시험결과표명，분리균주대두포고동납、두포새우납、두포곡송납、두포서람、두포부우、두포필우、아막서림、련매소、안곡남、낙불사성、좌불사성、은낙사성、잡나매소、안변청매소、아미잡성、련매소화아미잡성등다수약물민감。본연구수차보도료뢰겁씨보라비등사균가이인기포유자저엄중복사，제시재자저복사중응주의해균감염적진단、감측화방공。
    Investigation of a serious piglet disease with high mortality and typical diarrhoea yielded a bacte‐rial isolate identified as Providencia rettgeri based on the morphological characteristics ,physiological ,bio‐chemical characteristics and 16 S ribosomal DNA sequence analysis .The pathogenicity of this bacterial iso‐late was confirmed in mice ,and the same clinical signs were observed in 5‐day‐old piglets inoculated with Providencia rettgeri Guangxi 2013 at 5 .0 × 109 cfu .The same bacteria were recovered from the samples collected from died mice and piglets based on bacteria staining or biochemical tests results .Phylogenetic a‐nalysis showed the isolated strain had the nearest relationship with the P . rettgeri strains ,and shared 97 .3%‐99 .6% homology of 16 S rRNA with those strains .The drug resistance spectrum of the bacterium was also determined .The results indicated that the isolate is sensitive to 17 antibiotics out of the 29 antibi‐otics tested .The current study is the first to report a serious diarrheal disease in piglets infected by P . reggteri isolate ,which should be given more attention during surveillance and diagnostics .
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