CAJ | 학술논문

为了解牦牛NOBOX基因的特点，根据GenBank中公布的牛NOBOX基因序列设计2对引物，分两段扩增麦洼牦牛的NOBOX基因，然后测序并拼接。序列分析表明，扩增的牦牛NOBOX 基因大小为1975 bp ，其中开放阅读框全长1500 bp ，编码499个氨基酸，分子质量为53．12 ku。核苷酸序列同源性分析表明，NOBOX基因具有相对较高的保守性，牦牛与牛的NOBOX基因核苷酸序列同源性很高，为97％。在此基础上，借助Mega 5．0软件，采用N‐J算法构建了NOBOX氨基酸的系统进化树，分析了不同物种间的进化关系。牦牛NOBOX基因序列的成功克隆，为麦洼牦牛卵母细胞成熟及早期胚胎发育分子机制的研究及麦洼牦牛的遗传资源保护和育种提供了理论基础。
위료해모우NOBOX기인적특점，근거GenBank중공포적우NOBOX기인서렬설계2대인물，분량단확증맥와모우적NOBOX기인，연후측서병병접。서렬분석표명，확증적모우NOBOX 기인대소위1975 bp ，기중개방열독광전장1500 bp ，편마499개안기산，분자질량위53．12 ku。핵감산서렬동원성분석표명，NOBOX기인구유상대교고적보수성，모우여우적NOBOX기인핵감산서렬동원성흔고，위97％。재차기출상，차조Mega 5．0연건，채용N‐J산법구건료NOBOX안기산적계통진화수，분석료불동물충간적진화관계。모우NOBOX기인서렬적성공극륭，위맥와모우란모세포성숙급조기배태발육분자궤제적연구급맥와모우적유전자원보호화육충제공료이론기출。
To understand the character of NOBOX genes of yak ,two pairs of primers for NOBOX genes of yak was designed according to the published NOBOX genes sequence of Bos taurus in GenBank ,then se‐quencing and splicing ,and succeeded in cloning NOBOX genes of yak .The results of sequence analysis show that the length of the cloning NOBOX cDNA is 1 975 bp ,containing 1 500 bp open reading frame (ORF) .It encodes a peptide of 499 amino acids whose molecular mass is 53 .12 ku .Using sequence homol‐ogy analysis ,NOBOX genes are more conservative ,the gene sequence of yak and Bos taurus have the high‐est homology which is 97 .0% respectively .On this basis ,the experiment uses Mega 5 .0 software with NJ algorithm to construct a phylogenetic tree NOBOX protein ,analyzed of the evolutionary relationships a‐mong different species .The successful cloning of NOBOX gene sequence of yak provide important basis for the study of the molecular mechanism of Maiwa Yak oocyte maturation and its early embryonic develop‐ment ,as well as the protection of genetic resources and breeding .