CAJ | 학술논문

2株鸭源 H11N9亚型禽流感病毒的基因遗传进化及致病性分析
2주압원 H11N9아형금류감병독적기인유전진화급치병성분석
Genetic Analysis and Pathogenicity of 2 H11N9 Subtype Avian Influenza Isolates f rom Duck

万方数据

动物医学进展動物醫學進展 동물의학진전
PROGRESS IN VETERINARY MEDICINE
2015年 2期 25-28 ,共4页

谭丹%黄建龙%王昌建%何世成%王卫国%唐小明%朱春霞%范仲鑫%汪洪冰%刘道新%邓国华

담단%황건룡%왕창건%하세성%왕위국%당소명%주춘하%범중흠%왕홍빙%류도신%산국화

H11N9亚型禽流感%遗传进化%致病性 H11N9亞型禽流感%遺傳進化%緻病性
H11N9아형금류감%유전진화%치병성
H11N9 subtype Avian influenza virus%genetic evolution%pathogenicity

为了解从湖南省洞庭湖区鸭群中分离的2株 H11N9亚型禽流感病毒变异特点、进化规律及生物学特性，本研究对2株H11N9亚型禽流感病毒的HA、NA序列进行同源性和遗传进化分析，并用2株毒株对SPF鸡进行致病性试验。结果显示，本试验分离到2株 H11N9亚型禽流感毒株的 HA裂解位点均没有多个连续的碱性氨基酸插入，属于低致病性毒株；HA基因的受体结合位点均非常保守，具有典型的禽源性特征；NA基因序列与在周边国家野鸟中分离的H11N9亚型毒株的氨基酸同源性较高；鼻腔接种SPF鸡后，均能使鸡感染并通过喉头或泄殖腔排毒，但感染的鸡均不表现明显的临床症状，并且不能使同居鸡感染排毒。
위료해종호남성동정호구압군중분리적2주 H11N9아형금류감병독변이특점、진화규률급생물학특성，본연구대2주H11N9아형금류감병독적HA、NA서렬진행동원성화유전진화분석，병용2주독주대SPF계진행치병성시험。결과현시，본시험분리도2주 H11N9아형금류감독주적 HA렬해위점균몰유다개련속적감성안기산삽입，속우저치병성독주；HA기인적수체결합위점균비상보수，구유전형적금원성특정；NA기인서렬여재주변국가야조중분리적H11N9아형독주적안기산동원성교고；비강접충SPF계후，균능사계감염병통과후두혹설식강배독，단감염적계균불표현명현적림상증상，병차불능사동거계감염배독。
To study the genetic variation characteristics ,evolution and biological characteristics of two H11N9 subtype avian influenza virus isolated from ducks in the Dongting Lake area .We sequenced and an‐alyzed the HA and NA gene homology and genetic evolution ,and implemented pathogenicity tests in SPF chickens .The results show that two H11N9 subtype avian influenza virus belonged to low pathogenic avi‐an influenza virus ,because their cracking site did not inset some continuous alkaline amino acid .HA re‐ceptor binding site of the gene were very conservative ,with the typical characteristics of avian origin .The amino acid homology of NA genome were higher Similar with other H 11N9 subtype avian influenza virus i‐solated from wild birds in the neighboring countries .All SPF chickens were infected after intranasal inocu‐lation ,but the infected SPF chicken did not appear obvious clinical symptoms ,and cohabiting SPF chick‐ens were not infected .