动物医学进展
動物醫學進展
동물의학진전
PROGRESS IN VETERINARY MEDICINE
2015年
2期
131-133,134
,共4页
彭凌%杨旭夫%朱必凤%刘博婷
彭凌%楊旭伕%硃必鳳%劉博婷
팽릉%양욱부%주필봉%류박정
猪链球菌%16S rRNA%荚膜多糖%药敏试验
豬鏈毬菌%16S rRNA%莢膜多糖%藥敏試驗
저련구균%16S rRNA%협막다당%약민시험
Streptococcus suis%16S rRNA%Cps2J%drug sensitivity test
对来自广东韶关某猪场的疑似猪链球菌病病料进行分离鉴定,共分离到3株链球菌,对这3株链球菌进行了形态学观察、生化试验、16S rRNA基因序列分析、猪链球菌2型的毒力基因检测及药敏试验。结果表明,其形态、染色、生化特性均符合猪链球茵的特点,测序后Blast同源性分析,证实3株菌株均为猪链球菌,用猪链球菌2型Cps2J引物进行PCR扩增,获得预期的459 bp大小的目的片段。药敏试验结果表明,该菌对3种常用抗生素表现出敏感,对21种常用抗生素表现出耐药。
對來自廣東韶關某豬場的疑似豬鏈毬菌病病料進行分離鑒定,共分離到3株鏈毬菌,對這3株鏈毬菌進行瞭形態學觀察、生化試驗、16S rRNA基因序列分析、豬鏈毬菌2型的毒力基因檢測及藥敏試驗。結果錶明,其形態、染色、生化特性均符閤豬鏈毬茵的特點,測序後Blast同源性分析,證實3株菌株均為豬鏈毬菌,用豬鏈毬菌2型Cps2J引物進行PCR擴增,穫得預期的459 bp大小的目的片段。藥敏試驗結果錶明,該菌對3種常用抗生素錶現齣敏感,對21種常用抗生素錶現齣耐藥。
대래자엄동소관모저장적의사저련구균병병료진행분리감정,공분리도3주련구균,대저3주련구균진행료형태학관찰、생화시험、16S rRNA기인서렬분석、저련구균2형적독력기인검측급약민시험。결과표명,기형태、염색、생화특성균부합저련구인적특점,측서후Blast동원성분석,증실3주균주균위저련구균,용저련구균2형Cps2J인물진행PCR확증,획득예기적459 bp대소적목적편단。약민시험결과표명,해균대3충상용항생소표현출민감,대21충상용항생소표현출내약。
Isolation and identification of Streptococcus suis in Shaoguan Guangdong Province were conduc‐ted ,and 3 strains were obtained .morphology ,biochemistry test ,sequence analysis of 16S rRNA gene ,de‐tection of virulence genes of Streptococcus suis serotypes 2 and drug sensitivity test were done on them . The result of tests were as follows :their morphology ,dyeing characteristics ,biochemical characteristics were in coincidence with characteristics of Streptococcus suis .The sequences of 16S rRNA of 3 strains were obtained successfully .The sequences were compared with related sequence through online Blast in NCBI ,showing the 3 strains were Streptococcus suis .Thruogh this PCR ,which is based on the amplifica‐tion of the cps2J gene coding for the capsule of Streptococcus suis serotypes 2 ,A fragmentof 459 bp corre‐sponding to a part of the cps gene coding for the capsule was only obtained with Streptococcus suis sero‐types 2 strains as expecte .Drug susceptibility test results showed that the isolates were drugresistant to 21 kinds of antibiotic .