动物医学进展
動物醫學進展
동물의학진전
PROGRESS IN VETERINARY MEDICINE
2015年
2期
121-124,125
,共5页
王静%蒋万%何生虎%李志红%葛松%郭澍强
王靜%蔣萬%何生虎%李誌紅%葛鬆%郭澍彊
왕정%장만%하생호%리지홍%갈송%곽주강
急性毒性%腹腔注射%半数致死量(LD50)
急性毒性%腹腔註射%半數緻死量(LD50)
급성독성%복강주사%반수치사량(LD50)
acute toxicity%intraperitioneal injection%median lethal dose (LD50)
为了验证自制中药复方片剂的安全性，确定中草药配伍提取后制成的片剂的毒性，采用小鼠急性毒性试验方法测定小鼠半数致死量（LD50）。结果表明，复方中药片剂对小鼠腹腔的 LD50＝42884 mg／kg ，其Dmin＝10000 mg／kg ，Dmax ＝80000 mg／kg ，M LD＝16822 mg／kg ，LD50的95％可信限为35222．77～50545．23 mg／kg。注射剂量为47604 mg／kg的小鼠，其肝脏肿大，边缘呈暗红色，易碎，肾脏肿大不明显；而61742 mg／kg与80079 mg／kg的小鼠肝脏和肾脏均肿大，肝脏表面呈现紫红色，其切面呈暗红色，质地软易碎，肠系膜略有充血现象，其他脏器无眼观变化。根据毒理学评价标准，LD50＞10000 mg／kg属于无毒性物质，本试验证实中药复方片剂对小鼠安全无毒。
為瞭驗證自製中藥複方片劑的安全性，確定中草藥配伍提取後製成的片劑的毒性，採用小鼠急性毒性試驗方法測定小鼠半數緻死量（LD50）。結果錶明，複方中藥片劑對小鼠腹腔的 LD50＝42884 mg／kg ，其Dmin＝10000 mg／kg ，Dmax ＝80000 mg／kg ，M LD＝16822 mg／kg ，LD50的95％可信限為35222．77～50545．23 mg／kg。註射劑量為47604 mg／kg的小鼠，其肝髒腫大，邊緣呈暗紅色，易碎，腎髒腫大不明顯；而61742 mg／kg與80079 mg／kg的小鼠肝髒和腎髒均腫大，肝髒錶麵呈現紫紅色，其切麵呈暗紅色，質地軟易碎，腸繫膜略有充血現象，其他髒器無眼觀變化。根據毒理學評價標準，LD50＞10000 mg／kg屬于無毒性物質，本試驗證實中藥複方片劑對小鼠安全無毒。
위료험증자제중약복방편제적안전성，학정중초약배오제취후제성적편제적독성，채용소서급성독성시험방법측정소서반수치사량（LD50）。결과표명，복방중약편제대소서복강적 LD50＝42884 mg／kg ，기Dmin＝10000 mg／kg ，Dmax ＝80000 mg／kg ，M LD＝16822 mg／kg ，LD50적95％가신한위35222．77～50545．23 mg／kg。주사제량위47604 mg／kg적소서，기간장종대，변연정암홍색，역쇄，신장종대불명현；이61742 mg／kg여80079 mg／kg적소서간장화신장균종대，간장표면정현자홍색，기절면정암홍색，질지연역쇄，장계막략유충혈현상，기타장기무안관변화。근거독이학평개표준，LD50＞10000 mg／kg속우무독성물질，본시험증실중약복방편제대소서안전무독。
In order to evaluate the applied reliability and ascertain the toxicity of the self‐made traditional Chinese herbal compound medicine ,the experiment of acute toxicity and the determination of LD50 of the self‐made traditional Chinese herbal compound medicine was conducted in mice with different doses .The result showed that the LD50 of traditional Chinese herbal compound medicine was 42 884 mg per kg weight , the minority of the doses was 10 000 mg per kg weigh ,the maximum of the doses was 80 000 mg per kg weight ,the MLD was 16 822 mg per kg weight and the 95% confidence interval was from 35 222 .77 to 50 545 .23 mg per kg weight .Dissected symptom of died mice showed that the liver of 47 604 mg per kg weight was enlarged and fragiler with dark red of edge ,but the kindney's pathological change wasn't obvi‐ous .However ,the liver and kindney from 61 742 to 80 079 mg per kg weight were enlarged and its surface was purple ,the mesentery bleeding slightly ,but the other organs didn't show obvious pathological chan‐ges .According to the toxicological evaluation standard ,when LD50 is greater than 10 000 mg per kg weight ,the medicine belongs to the non‐toxic matter ,the results confirmed that the self‐made traditional Chinese herbal compound medicine was safe and non‐toxic for mice .
