安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2015年
1期
37-40,41
,共5页
闵曦%夏荣%孙磊%徐基亮%孙子环
閔晞%夏榮%孫磊%徐基亮%孫子環
민희%하영%손뢰%서기량%손자배
TiO2 纳米管%阳极氧化%紫外光催化%亲水性%骨髓基质干细胞
TiO2 納米管%暘極氧化%紫外光催化%親水性%骨髓基質榦細胞
TiO2 납미관%양겁양화%자외광최화%친수성%골수기질간세포
TiO2 nanotubes%anodization%ultraviolet catalysis%hydrophilicity%bone marrow stromal cells
目的:研究紫外光照射对老化TiO2纳米管表面理化性质和生物活性的影响。方法两步阳极氧化后的钛片避光保存8周,使其充分老化,紫外光照射48 h;利用场发射扫描电镜(FESEM)、X射线光电子能谱(XPS)、接触角测量仪分析新鲜、老化及紫外光照射组钛片表面微观结构、化学元素和接触角变化;以小鼠骨髓未分化间充质干细胞( MSCs)为细胞株,检测各组钛表面对细胞黏附、增殖及分化的影响,评价各组间生物学差异。结果 FESEM显示紫外光照射未改变钛表面TiO2纳米管形态, XPS结果显示老化组表面C元素含量显著增高,经紫外光照射后恢复到新鲜组水平,接触角检测显示老化组表面呈疏水性,紫外光照射组表面成超亲水性。体外细胞学实验显示,紫外光照射后钛表面有利于细胞黏附、增殖和分化。结论紫外光照射可去除钛表面碳氢化合物污染,提高表面亲水性,延缓时间因素造成的TiO2纳米管表面的生物活性降低。
目的:研究紫外光照射對老化TiO2納米管錶麵理化性質和生物活性的影響。方法兩步暘極氧化後的鈦片避光保存8週,使其充分老化,紫外光照射48 h;利用場髮射掃描電鏡(FESEM)、X射線光電子能譜(XPS)、接觸角測量儀分析新鮮、老化及紫外光照射組鈦片錶麵微觀結構、化學元素和接觸角變化;以小鼠骨髓未分化間充質榦細胞( MSCs)為細胞株,檢測各組鈦錶麵對細胞黏附、增殖及分化的影響,評價各組間生物學差異。結果 FESEM顯示紫外光照射未改變鈦錶麵TiO2納米管形態, XPS結果顯示老化組錶麵C元素含量顯著增高,經紫外光照射後恢複到新鮮組水平,接觸角檢測顯示老化組錶麵呈疏水性,紫外光照射組錶麵成超親水性。體外細胞學實驗顯示,紫外光照射後鈦錶麵有利于細胞黏附、增殖和分化。結論紫外光照射可去除鈦錶麵碳氫化閤物汙染,提高錶麵親水性,延緩時間因素造成的TiO2納米管錶麵的生物活性降低。
목적:연구자외광조사대노화TiO2납미관표면이화성질화생물활성적영향。방법량보양겁양화후적태편피광보존8주,사기충분노화,자외광조사48 h;이용장발사소묘전경(FESEM)、X사선광전자능보(XPS)、접촉각측량의분석신선、노화급자외광조사조태편표면미관결구、화학원소화접촉각변화;이소서골수미분화간충질간세포( MSCs)위세포주,검측각조태표면대세포점부、증식급분화적영향,평개각조간생물학차이。결과 FESEM현시자외광조사미개변태표면TiO2납미관형태, XPS결과현시노화조표면C원소함량현저증고,경자외광조사후회복도신선조수평,접촉각검측현시노화조표면정소수성,자외광조사조표면성초친수성。체외세포학실험현시,자외광조사후태표면유리우세포점부、증식화분화。결론자외광조사가거제태표면탄경화합물오염,제고표면친수성,연완시간인소조성적TiO2납미관표면적생물활성강저。
Objective To study the impact of UV radiation on the physicochemical properties and biological activity of aging TiO2 nanotube surface. Methods Titanium plates treated by two-step anodization were stored in dark for eight weeks, sufficient to aging, and irradiated by UV for 48 h. Field emission scanning electron microscopy ( FESEM) , X-ray photoelectron spectroscopy ( XPS) and contact angle measurement were used to analyze the mi-crostructure, chemical elements and the contact angle of the surface of the fresh, aging, UV irradiation groups, re-spectively. Mouse bone marrow mesenchymal stem cells (MSCs) as cell lines were cultured on the treated titanium plates to determine the effect of modified titanium surface on the cell adhesion, proliferation and differentiation, fur-ther to evaluate biological differences among the three groups. Results FESEM displayed UV irradiation did not change the morphology of TiO2 nanotubes on the titanium surface. XPS showed that C elements on the surface of ag-ing group significantly increased after UV irradiation but restored to the level of fresh group by UV irritation. The contact angle analysis showed that the surface of age group was hydrophobic while the surface of UV irradiated group was superhydrophilic. In vitro cell culture showed that UV irritation was conducive to cell adhesion, proliferation and differentiation. Conclusion UV radiation can remove hydrocarbon contamination on surface of titanium, im-prove the surface hydrophilicity, and delay the bioactive decrease of titanium-based TiO2 nanotube surface by time factors.