CAJ | 학술논문

目的：探讨老龄帕金森病( PD)模型小鼠行为学改变及黑质纹状体系统氧化应激相关基因的表达差异。方法10月龄快速老化P8系( SAMP8)小鼠16只,随机均分为对照组和模型组。模型组小鼠背部皮下注射1-甲基-4-苯基-1,2,3,6-四氢吡啶( MPTP)制成急性损伤模型,对照组小鼠给予同量生理盐水。给药后72 h进行行为学测试,高效液相色谱法检测黑质多巴胺( DA)含量,PCR Array检测其纹状体氧化应激相关基因的表达。结果与对照组相比,模型组小鼠DA水平下降,行为学成绩下降,差异有统计学意义( P<0．01)；环氧化酶-2、可诱导型一氧化氮合酶2、还原型烟酰胺腺嘌呤二核苷酸磷酸( NADPH)氧化酶基因表达上调；而谷胱甘肽过氧化物酶3、6、8等9种基因明显下调(改变倍数>2)。结论老龄PD模型小鼠氧化应激相关的基因发生上调或下调,导致氧化应激反应的发生。
목적：탐토노령파금삼병( PD)모형소서행위학개변급흑질문상체계통양화응격상관기인적표체차이。방법10월령쾌속노화P8계( SAMP8)소서16지,수궤균분위대조조화모형조。모형조소서배부피하주사1-갑기-4-분기-1,2,3,6-사경필정( MPTP)제성급성손상모형,대조조소서급여동량생리염수。급약후72 h진행행위학측시,고효액상색보법검측흑질다파알( DA)함량,PCR Array검측기문상체양화응격상관기인적표체。결과여대조조상비,모형조소서DA수평하강,행위학성적하강,차이유통계학의의( P<0．01)；배양화매-2、가유도형일양화담합매2、환원형연선알선표령이핵감산린산( NADPH)양화매기인표체상조；이곡광감태과양화물매3、6、8등9충기인명현하조(개변배수>2)。결론노령PD모형소서양화응격상관적기인발생상조혹하조,도치양화응격반응적발생。
Objective To explore the behavior changes and oxidative stress related gene expression difference in nigro-striatal system of aging Parkinson’ s disease model mice. Methods Senescence-accelerated mouse prone 8 ( SAMP8) aged 10-month old in sum of 16 were randomly divided into control group and model group. Acute injury was made by subcutaneous injections of 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) to mice in model group, while a corresponding volume of saline was given to the control. 72 hours after injection, all the animals were tested by the behavioral test. Dopamine ( DA) contents in nigro were measured by a high performance liquid chromatography with electochemical detector, and the oxidative stress related genes expression was detected by pol-ymerase chain reaction array ( PCR Array) . Results Compared with the control group, the DA levels and behav-ioral performance were both remarkably decreased in model group ( P<0. 01 ) . Cyclooxygenase-2 , inducible Nitric oxide synthase 2, reduced form of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase were up-regula-ted, while 9 genes such as glutathione peroxidase 3,6,8 were all down-regulated significantly (fold change>2). Conclusion Up-or down-regulation of oxidative stress related genes may result in the occurence of oxidative stress in aging PD model mice.