CAJ | 학술논문

建立2,4,6-三硝基苯磺酸( TNBS)小鼠克罗恩病( CD)模型,通过观察小鼠每日表现、存活率、疾病活动指数( DAI)、结肠肉眼观和组织学变化及肠道纤维化情况,筛选出造模所需最佳乙醇浓度。各模型组小鼠均不同程度出现CD的一般表现。随着乙醇浓度增加,小鼠 DAI评分、肠道病理及纤维化评分逐渐增高,以 TNBS/50%乙醇组最为显著,最类似人类CD病理改变。50%为造模所需最佳乙醇浓度,其配比2．0 mg TNBS诱导的模型成模率高且小鼠生存率适中,较适宜用作CD发病机制研究和新靶点的探索。
건립2,4,6-삼초기분광산( TNBS)소서극라은병( CD)모형,통과관찰소서매일표현、존활솔、질병활동지수( DAI)、결장육안관화조직학변화급장도섬유화정황,사선출조모소수최가을순농도。각모형조소서균불동정도출현CD적일반표현。수착을순농도증가,소서 DAI평분、장도병리급섬유화평분축점증고,이 TNBS/50%을순조최위현저,최유사인류CD병리개변。50%위조모소수최가을순농도,기배비2．0 mg TNBS유도적모형성모솔고차소서생존솔괄중,교괄의용작CD발병궤제연구화신파점적탐색。
Crohn’s disease(CD)mice model was induced by 2, 4, 6-trinitrobenzene sulfonic acid(TNBS). The daily performance, survival rate, disease activity index ( DAI) , colon morphologic and histologic changes and in-testinal fibrosis degree of mice were observed in order to investigate the optimal ethanol concentration. Mice in mod-el groups all performed general symptoms of CD in different degrees. The grades of mice DAI, histology and intesti-nal fibrosis increased gradually with the increase of ethanol concentrations, and the TNBS/50% ethanol group was most significant which was most similar to human CD pathological changes. An ethanol concentration of 50% for mice was chosen for an optimal experimental concentration since the group had the highest success rate of modeling when matched with 2. 0 mg TNBS and showed appropriate survival rate, which was more suitable for the investiga-tion of pathogenesis and new therapeutic targets of CD.