CAJ | 학술논문

目的：建立一种简易的测定联合服用苦参素和阿德福韦酯成人血清中苦参素和阿德福韦酯含量方法。方法采用Shim-pack VP-ODS C18色谱柱（250 mm ×4．6 mm，5μm），流动相为甲醇∶水（70∶30），流速为1．1 mL·min －1，检测波长为229 nm。结果苦参素保留时间4．6 min 左右，标准曲线的线性范围为0．0125～2 mg·L －1，r ＝0．9992。阿德福韦酯保留时间7．3 min 左右，标准曲线的线性范围为0．0125～0．75 mg·L －1，r ＝0．9991，苦参素和阿德福韦酯的日间、日内变异系数均在7％以下，回收率＞92％。结论该法简便，灵敏，快速，可用于苦参素和阿德福韦酯临床联用治疗的成人血药浓度的监测。
목적：건립일충간역적측정연합복용고삼소화아덕복위지성인혈청중고삼소화아덕복위지함량방법。방법채용Shim-pack VP-ODS C18색보주（250 mm ×4．6 mm，5μm），류동상위갑순∶수（70∶30），류속위1．1 mL·min －1，검측파장위229 nm。결과고삼소보류시간4．6 min 좌우，표준곡선적선성범위위0．0125～2 mg·L －1，r ＝0．9992。아덕복위지보류시간7．3 min 좌우，표준곡선적선성범위위0．0125～0．75 mg·L －1，r ＝0．9991，고삼소화아덕복위지적일간、일내변이계수균재7％이하，회수솔＞92％。결론해법간편，령민，쾌속，가용우고삼소화아덕복위지림상련용치료적성인혈약농도적감측。
Objective To establish a method for determination of oxymatrine and adefovir dipivoxil in human serum.Methods RP-HPLC method was used,with C18 column at the standard of 250 mm ×4.6 mm,methanol-water(70∶30)as mobile phase.The flow rate was 1.1 mL·min -1 ,with the detection wavelength at 229 nm.Results The reserve time of oxymatrine in human blood serum was 4. 6 min.The linearity range of standard curve was 0.0125 ~2 mg·L -1 (r =0.999 2).The reserve time of adefovir dipivoxil in human blood serum was 7.3 min.The linearity range of standard curve was 0.012 5 ~0.75 mg·L -1 (r =0.999 1).The average recoveries of oxymatrine and adefovir dipivoxil were both above 92%.The intraday and extraday CVs were below 7%.Conclusions The method is simple,sensitive,rapid and can be used for monitoring and analysis of oxymatrine and adefovir dipivoxil in human blood serum.