CAJ | 학술논문

目的：探讨核因子-κB(NF-κB)沉默对鼻咽癌5-8F细胞的增殖抑制作用。方法构建NF-κB p65沉默重组质粒表达载体pGenesil-1.2-NF-κB和阴性对照重组质粒载体pGenesil-1.2-HK,应用转染试剂将重组质粒表达载体转染人鼻咽癌5-8F细胞,采用Western blot法检测NF-κB p65的表达,采用MTT及流式细胞仪分析细胞增殖和细胞周期。结果 NF-κB p65沉默抑制鼻咽癌5-8F细胞增殖,抑制率为(58．43±1．24)%,与对照组细胞抑制率(0)和阴性对照组细胞抑制率(17．89±4．13)%比较,差异有统计学意义( P<0．05)。 NF-κB沉默组S期细胞为(42．27±0．39)%,与空白对照组(30．83±1．36)%和阴性对照组(34．88±0．85)%比较,差异有统计学意义( P<0．05)。结论 NF-κB p65沉默抑制鼻咽癌5-8F细胞增殖,其可能是通过S期细胞阻滞起作用。
목적：탐토핵인자-κB(NF-κB)침묵대비인암5-8F세포적증식억제작용。방법구건NF-κB p65침묵중조질립표체재체pGenesil-1.2-NF-κB화음성대조중조질립재체pGenesil-1.2-HK,응용전염시제장중조질립표체재체전염인비인암5-8F세포,채용Western blot법검측NF-κB p65적표체,채용MTT급류식세포의분석세포증식화세포주기。결과 NF-κB p65침묵억제비인암5-8F세포증식,억제솔위(58．43±1．24)%,여대조조세포억제솔(0)화음성대조조세포억제솔(17．89±4．13)%비교,차이유통계학의의( P<0．05)。 NF-κB침묵조S기세포위(42．27±0．39)%,여공백대조조(30．83±1．36)%화음성대조조(34．88±0．85)%비교,차이유통계학의의( P<0．05)。결론 NF-κB p65침묵억제비인암5-8F세포증식,기가능시통과S기세포조체기작용。
Objective To evaluate the inhibition effects of nuclear factor-κB( NF-κB) silencing in the nasopharyn-geal carcinoma 5-8F cells. Methods Constructing the NF-κB SiRNA expression vector pGenesil-1. 2-NF-κB and emptor pGenesil-1. 2-HK,and then they were transfected into nasopharyngeal carcinoma 5-8F cells. Western blot was used to detect expression of NF-κB p65 protein. MTT and flow cytometry were used to evaluate the proliferation inhibition and the cell cycle arrest in 5-8F cells respectively. Results NF-κB p65 silencing could inhibit the pro-liferation of nasopharyngeal carcinoma 5-8F cells and the inhibition rate was (58. 43 ± 1. 24)%,0% in the control group and (17. 89 ± 4. 13)% in the empty vector control group, and the difference was statistically significant ( P<0. 05). The cell number of S phase was (42. 27 ± 0. 39)% in NF-κB silencing group, (30. 83 ± 1. 36)% in the control group and (34. 88 ± 0. 85)% in the empty vector control group, and the difference was statistically signifi-cant ( P<0. 05 ) . Conclusion The NF-κB p65 knockdown decrease cell proliferation in nasopharyngeal carcino-ma 5-8F cells, maybe due to cell cycle arresting in S phase.