CAJ | 학술논문

目的：探讨三酸散的制备方法和建立 HPLC 法测定三酸散中苯甲酸和水杨酸的含量方法，为三酸散质量控制提供有效的分析手段。方法色谱柱：Eclipcy plus C18（250 mm ×4．6 mm，5μm）；流动相：甲醇—0．05 mol·mL －1磷酸二氢钾溶液（用磷酸调节 pH 值至4．0）（1∶1）；检测波长：232 nm；流速：1．0 mL·min －1；柱温：室温。结果苯甲酸和水杨酸的线性范围分别是5～30 mg·L －1和10～60 mg·L －1，回归方程分别是 A ＝109287C ＋37431（r ＝0．9943）和 A ＝63894C ＋4890．2（r ＝0．9956）。苯甲酸和水杨酸的平均回收率分别是100．2％（RSD ＝1．25％）和99．74％（RSD ＝1．43％）。结论该方法简单，结果准确可靠，可用于三酸散中苯甲酸和水杨酸的含量测定。
목적：탐토삼산산적제비방법화건립 HPLC 법측정삼산산중분갑산화수양산적함량방법，위삼산산질량공제제공유효적분석수단。방법색보주：Eclipcy plus C18（250 mm ×4．6 mm，5μm）；류동상：갑순—0．05 mol·mL －1린산이경갑용액（용린산조절 pH 치지4．0）（1∶1）；검측파장：232 nm；류속：1．0 mL·min －1；주온：실온。결과분갑산화수양산적선성범위분별시5～30 mg·L －1화10～60 mg·L －1，회귀방정분별시 A ＝109287C ＋37431（r ＝0．9943）화 A ＝63894C ＋4890．2（r ＝0．9956）。분갑산화수양산적평균회수솔분별시100．2％（RSD ＝1．25％）화99．74％（RSD ＝1．43％）。결론해방법간단，결과준학가고，가용우삼산산중분갑산화수양산적함량측정。
Objective To explore the preparation method of tri-acids powder and to establish an HPLC method for the determination of benzoic acid and salicylic acid in tri-acids powder.Methods Diamonsil C18 column was used with methanol -0.05mol·ml -1 KH2 PO4 solution (adjusted to pH 4 with phosphoric acid)(1∶1)as a mobile phase.Detective wavelength was 232 nm.The flow rate was 1.0 mL·min -1 .Results The regress equation of benzoic acid and salicylic acid were A =1 092 87C +37 431(r =0.994 3)and A =63 894C +4 890.2(r =0.995 6),respectively.The linear ranges of benzoic acid and salicylic acid were 5 ~30 and 10 ~60 mg·L -1 ,re-spectively.The average recoveries of benzoic acid and salicylic acid were 100.2%(RSD =1.25%)and 99.74%(RSD =1.43%),re-spectively.Conclusions The method is simple and reliable,which can be used to determine the concentration of benzoic acid and sali-cylic acid in tri-acids powder.