CAJ | 학술논문

目的：探讨麝香醒脑滴丸（Shexiang Xingnao dropping pills，SXD）对大鼠脑缺血再灌注损伤的保护作用及其机制。方法线栓法建立大鼠局灶性脑缺血（MCAO）再灌注模型，按 Bederson 的方法对 MCAO 再灌注0、4、8、22 h 时大鼠的神经功能损伤进行评分；测定脑梗死百分比、脑指数、脑含水量；检测血浆中血小板聚集率和脑组织超氧化物歧化酶（SOD）、乳酸脱氢酶（LDH）活性以及丙二醛（MDA）含量；HE 染色观察脑组织病理变化。结果与模型组比较，SXD（0．42、0．84 g·kg －1）对MCAO 再灌注大鼠的神经功能损伤评分有一定的改善作用，能减少脑梗死百分比、脑含水量、脑指数和血小板聚集率，提高脑组织 SOD 和 LDH 活性，降低脑组织中 MDA 含量，减轻脑组织皮质神经元的损伤。结论SXD 对脑缺血再灌注损伤具有一定的保护作用，其机制可能与抗氧自由基损伤及抑制血小板聚集等有关。
목적：탐토사향성뇌적환（Shexiang Xingnao dropping pills，SXD）대대서뇌결혈재관주손상적보호작용급기궤제。방법선전법건립대서국조성뇌결혈（MCAO）재관주모형，안 Bederson 적방법대 MCAO 재관주0、4、8、22 h 시대서적신경공능손상진행평분；측정뇌경사백분비、뇌지수、뇌함수량；검측혈장중혈소판취집솔화뇌조직초양화물기화매（SOD）、유산탈경매（LDH）활성이급병이철（MDA）함량；HE 염색관찰뇌조직병리변화。결과여모형조비교，SXD（0．42、0．84 g·kg －1）대MCAO 재관주대서적신경공능손상평분유일정적개선작용，능감소뇌경사백분비、뇌함수량、뇌지수화혈소판취집솔，제고뇌조직 SOD 화 LDH 활성，강저뇌조직중 MDA 함량，감경뇌조직피질신경원적손상。결론SXD 대뇌결혈재관주손상구유일정적보호작용，기궤제가능여항양자유기손상급억제혈소판취집등유관。
Objective To explore the effects of Shexiang Xingnao dropping pills (SXD)on cerebral ischemia/reperfusion injury and its mechanisms.Methods Middle cerebral artery occlusion (MCAO)was used to make focal cerebral ischemia/reperfusion model by in-travascular nylon filament occlusion.The neurological function score,the percentage of cerebral infarction,the content of cerebral water, the cerebral index and the pathomorphology of brain were used to evaluate the protective effects of SXD.The activity of SOD,LDH and the content of MDA in brain tissue was detected by kit. And the rate of platelet aggregation was detected in vitro.Results Compared with model group,SXD (0.42,0.84 g·kg -1 )could decrease the neurological score,reduce the percentage of cerebral infarction,de-crease the content of cerebral water and the cerebral index,and inhibit platelet aggregation.The results showed that SXD could signifi-cantly increase the SOD and LDH activity,and reduce the MDA content in brain tissue.And the pathological results showed that SXD could relieve neuronal injury in cortex of brain after ischemia reperfusion.Conclusions The SXD has protective effects on cerebral is-chemia reperfusion injury and its mechanisms may be related to its antioxidation and inhibition of platelet aggregation.