CAJ | 학술논문

目的：研究复发性流产小鼠蜕膜血管内皮生长因子（VEGF）、微血管密度（MVD）的表达及补肾健脾中药干预，从血管的角度探讨中药补肾安胎冲剂的保胎作用机制，为临床上运用中医药治疗自然流产的患者提供依据，并为今后进一步的实验研究奠定基础。方法建立复发性流产小鼠模型，随机分为正常组（A）、模型组（B）、西药（黄体酮）组（C）、中药（补肾安胎冲剂）组（D）；从妊娠第一天开始，A 组、B 组给予蒸馏水灌胃；C 组予黄体酮胶囊（0．4 mg·kg －1·d －1）灌胃；D 组予补肾安胎冲剂（3．0 g·kg －1·d －1）灌胃，各组均15 d。进行不同的干预，比较蜕膜中 VEGF 及 MVD 表达的差异及中药干预结果。结果B 组流产率高于 A 组及 C 组、D 组（P ＜0．01）；与 A 组比较，B 组中 VEGF、MVD 表达减少（P ＜0．05或 P ＜0．01）；与 B 组比较， C 组及 D 组中 VEGF、MVD 表达增加（P ＜0．05或 P ＜0．01）。结论复发性流产小鼠蜕膜存在 VEGF、MVD 表达不足，补肾健脾中药能上调蜕膜中 VEGF、MVD 的表达，改善血管形成，促进胚胎发育。
목적：연구복발성유산소서세막혈관내피생장인자（VEGF）、미혈관밀도（MVD）적표체급보신건비중약간예，종혈관적각도탐토중약보신안태충제적보태작용궤제，위림상상운용중의약치료자연유산적환자제공의거，병위금후진일보적실험연구전정기출。방법건립복발성유산소서모형，수궤분위정상조（A）、모형조（B）、서약（황체동）조（C）、중약（보신안태충제）조（D）；종임신제일천개시，A 조、B 조급여증류수관위；C 조여황체동효낭（0．4 mg·kg －1·d －1）관위；D 조여보신안태충제（3．0 g·kg －1·d －1）관위，각조균15 d。진행불동적간예，비교세막중 VEGF 급 MVD 표체적차이급중약간예결과。결과B 조유산솔고우 A 조급 C 조、D 조（P ＜0．01）；여 A 조비교，B 조중 VEGF、MVD 표체감소（P ＜0．05혹 P ＜0．01）；여 B 조비교， C 조급 D 조중 VEGF、MVD 표체증가（P ＜0．05혹 P ＜0．01）。결론복발성유산소서세막존재 VEGF、MVD 표체불족，보신건비중약능상조세막중 VEGF、MVD 적표체，개선혈관형성，촉진배태발육。
Objective To investigate the effect of Bushen Jianpi Herbs on the expression of VEGF and MVD in decidual of RSA rat, From the aspects of vascular,we explore the further mechanism of Bushen Antai Granules on pregnancy and fetal protection.These can provide an important reference for the clinical reasearchs to develop a treatment program about the TCMtreatment of patients with early spontaneous abortion,and lay the theoretical foundation for further experiments in the future.Methods Duplicate RSA rat,and the rats were randomized into normal group(A),model group(B),progesterone group(C),Bushen Antai Granule group(D).From the first day of pregnancy,the normal group and the model group were fed with distilled water.Western medicine group was given progesterone(0.4 mg·kg -1 ·d -1 ).The Chinese medicine group was treated with Bushen Antai Granule(3.0 g·kg -1 ·d -1 ).Respective interventions lasted fifteen days in each group.Different expressions of VEGF and MVD in decidual were compared and the effect of traditional Chi-nese medicine was investigated on each group.Results Compared with the A,C,D group,the abortion rate was clearly higher in B group(P <0.01).Compared with the A group,the expressions of VEGF and MVD were clearly lower in B group(P <0.05 or P <0.01).Compared with the B group,the expressions of VEGF and MVD were clearly higher in both C group and D group(P <0.05 or P<0.01).Conclusions RSA rats have insufficient expressions of VEGF and MVD in deciduas.Bushen jianpi herbs can increase the val-ues of VEGF and MVD in deciduas so as to improve the formation of blood vessel and ultimately promote the development of the embryo.