CAJ | 학술논문

为了对双孢蘑菇褐腐病的科学生态防控提供理论依据，测定了4株形态差异较大的有害疣孢霉菌（GS 0036，HN 0015，SD 007，WH 001）在常见蘑菇煎汁培养基、不同光质条件和不同浓度盐溶液条件下的菌丝生长速度、厚垣孢子产生量、分生孢子萌发情况和耐盐性。结果表明：除灵芝煎汁外，6种常见蘑菇煎汁培养基对供试菌株厚垣孢子的产生均优于 PDA 培养基；不同光质对有害疣孢霉菌影响不同，SD007菌株在红光下菌丝生长速度最快，其他3种菌株均在黑／白交替光质下菌丝生长最快，短波光（蓝光）能抑制有害疣孢霉菌丝的生长及厚垣孢子的产生；4株有害疣孢霉的菌丝生长量和厚垣孢子产生量随着 NaCl 浓度的升高均明显下降，NaCl 溶液对 HN 0015抑制效果最明显，当 NaCl 浓度在24 g／L 时所有有害疣孢霉菌菌株的分生孢子几乎不萌发。
위료대쌍포마고갈부병적과학생태방공제공이론의거，측정료4주형태차이교대적유해우포매균（GS 0036，HN 0015，SD 007，WH 001）재상견마고전즙배양기、불동광질조건화불동농도염용액조건하적균사생장속도、후원포자산생량、분생포자맹발정황화내염성。결과표명：제령지전즙외，6충상견마고전즙배양기대공시균주후원포자적산생균우우 PDA 배양기；불동광질대유해우포매균영향불동，SD007균주재홍광하균사생장속도최쾌，기타3충균주균재흑／백교체광질하균사생장최쾌，단파광（람광）능억제유해우포매균사적생장급후원포자적산생；4주유해우포매적균사생장량화후원포자산생량수착 NaCl 농도적승고균명현하강，NaCl 용액대 HN 0015억제효과최명현，당 NaCl 농도재24 g／L 시소유유해우포매균균주적분생포자궤호불맹발。
In order to provide a theoretical basis for scientific ecological prevention and control of brown rot on agaricus bisporus,mycelial growth speed,chlamydospore yield,conidium germination status and salt tolerance of four M.perniciosa strains with great morphological differences,including GS 0036, HN 0015,SD 007 and WH 001,were studied under various conditions of culture medium,light quality and saline solution.Results:Chlamydospore yield on six common mushroom extract media except lucid ganoderma were all higher than PDA culture medium;SD 007 mycelia grew the fastest under red light,and the other three grew the fastest with the alternation of day and night,short-wave light(blue light)could inhibit mycelial growth and conidium germination; Hypha growth rate and chlamydospore yield significantly decreased with NaCl concentration increasing.NaCl solution inhibited HN0015 the most significantly.All conidia almost did not germinate when NaCl concentration was 24 g/L.