CAJ | 학술논문

以猴面花基因组序列为试材，通过 psRobot 网站进行茎环结构预测，应用 blast-2．2．27＋软件与 rfam 数据库和 pfam 数据库比对后去除非 miRNA 序列，应用 bioedit 分析 miRNA 序列及前体序列的碱基组成特点。结果表明：猴面花基因组中共发现105条成熟 miRNA 序列，分别属于49个不同 miRNA 家族；miRNA 序列和其侧翼序列都存在碱基偏倚现象，miRNA5′端第1碱基和第19碱基尿嘧啶和胞嘧啶出现的频率分别为67．6％和49．5％；93个 miRNA 预测到了靶基因，其中有64个靶向转录因子。
이후면화기인조서렬위시재，통과 psRobot 망참진행경배결구예측，응용 blast-2．2．27＋연건여 rfam 수거고화 pfam 수거고비대후거제비 miRNA 서렬，응용 bioedit 분석 miRNA 서렬급전체서렬적감기조성특점。결과표명：후면화기인조중공발현105조성숙 miRNA 서렬，분별속우49개불동 miRNA 가족；miRNA 서렬화기측익서렬도존재감기편의현상，miRNA5′단제1감기화제19감기뇨밀정화포밀정출현적빈솔분별위67．6％화49．5％；93개 miRNA 예측도료파기인，기중유64개파향전록인자。
Taking genome sequence of M.gutatus as material,prediction of stem loop structure of pre-miRNAs was run on the web-based software psRobot,then non-miRNA sequences were removed by BLAST search against rfam and pfam database.The characteristics of base composition was analyzed by bioedit software in miRNA sequences and their precursors.The result showed that there are 105 mature sequences in M.gutatus which belong to 49 different miRNA familes.The base bias phenomenon has been found in miRNA sequence and their flanking sequences.The frequency of uracil and cytosine is respectively 67.6% and 49.5% in the first base and nineteenth base from miRNA 5′end.The target genes has been found in 93 miRNAs,in which 64 miRNAs regulate transcription factors.