CAJ | 학술논문

为更好地开发利用红！木，采用乙醇浸提的方法获得红！木嫩叶、老叶及花中的总黄酮，并测定其黄酮含量和总抗氧化活性，以及清除超氧阴离子、羟基自由基和 DPPH 自由基的能力。结果表明：红！木花、嫩叶和老叶中的总黄酮含量分别为7．44％、4．75％、3．82％。各部位醇提物浓度在0．00～0．35 mg／mL时，均有抗氧化活性，花的活性最高；浓度在0．0～0．4 mg／mL 时，抑制邻苯三酚自氧化速率和羟基自由基的清除率低于20％；当浓度大于1．5μg／mL 时，对 DPPH 的清除率均大于50％，老叶在7．5μg／mL时的清除率可达90％，各部位均表现出一定的抗氧化活性，且随浓度的增加，抗氧化能力增强。
위경호지개발이용홍！목，채용을순침제적방법획득홍！목눈협、로협급화중적총황동，병측정기황동함량화총항양화활성，이급청제초양음리자、간기자유기화 DPPH 자유기적능력。결과표명：홍！목화、눈협화로협중적총황동함량분별위7．44％、4．75％、3．82％。각부위순제물농도재0．00～0．35 mg／mL시，균유항양화활성，화적활성최고；농도재0．0～0．4 mg／mL 시，억제린분삼분자양화속솔화간기자유기적청제솔저우20％；당농도대우1．5μg／mL 시，대 DPPH 적청제솔균대우50％，로협재7．5μg／mL시적청제솔가체90％，각부위균표현출일정적항양화활성，차수농도적증가，항양화능력증강。
To preferably develop and utilize L.chinense,total flavonoids were gained from tender leaves,old leaves and flowers by ethanol extraction method,then the flavonoid content and antioxidant activities were detected,as well as the scavenging activity of superoxide anion,hydroxyl radical and DPPH radical.Results:Total flavonoid contents were respectively 7.44%,4.75% and 3.82% in flowers,tender leaves and old leaves,which was the highest in flowers.Antioxidant activity could be detected when the concentration was 0~0.35 mm/mL,and it was the highest in flowers;The scavenging rate of pyrogallic acid and hydroxyl radical was lower than 20% when the concentration was 0~0.4 mg/mL;Scavenging rate of DPPH radical was over 50% when the concentration was over 1.5 μg/mL,and it could be over 90%when the concentration was 7.5 μg/mL in old leaves,antioxidant activity appeared in various parts,and it increased with concentration increasing.