心房钠尿肽基因敲除小鼠的鉴定
심방납뇨태기인고제소서적감정
Identification of Atrial Natriuretic Peptide Gene Knockout (ANP-/-)Mice
  • 万方数据
    动物医学进展 동물의학진전
    PROGRESS IN VETERINARY MEDICINE
    2015年 1期 71-74 ,共4页

    杨扬%李斌%叶杰%亓翠玲%何晓东%李江超%章倩倩%黄韧%张钰%王丽京

    양양%리빈%협걸%기취령%하효동%리강초%장천천%황인%장옥%왕려경

    心房钠尿肽%转基因小鼠 心房鈉尿肽%轉基因小鼠
    심방납뇨태%전기인소서
    atrial natriuretic peptide%transgenic animal model
    研究心房钠尿肽基因敲除小鼠（ANP－／－小鼠）的生物学及病理学特性。 ANP－／－小鼠与C57BL／6小鼠饲养于SPF级环境中，观察小鼠的繁育状况及其生物学特性，选取合适时间剖杀小鼠，通过HE染色观察脏器形态及组织结构。ANP－／－小鼠的繁殖周期比C57BL／6长，产仔率较低；ANP－／－小鼠肝脏、肺脏、脾脏较C57BL／6小鼠的有明显炎症反应，但是C57BL／6小鼠未见明显的病理改变。提示ANP基因的缺失可能与炎症发生有关。说明ANP－／－小鼠繁殖较慢、产仔率低，且正常繁殖的普通小鼠不会引起器官的病理学改变。但作为一种在心血管、代谢疾病上的动物模型，并与炎症甚至肿瘤有可能的未知联系的转基因动物，其很有研究和探索价值。
    연구심방납뇨태기인고제소서（ANP－／－소서）적생물학급병이학특성。 ANP－／－소서여C57BL／6소서사양우SPF급배경중，관찰소서적번육상황급기생물학특성，선취합괄시간부살소서，통과HE염색관찰장기형태급조직결구。ANP－／－소서적번식주기비C57BL／6장，산자솔교저；ANP－／－소서간장、폐장、비장교C57BL／6소서적유명현염증반응，단시C57BL／6소서미견명현적병리개변。제시ANP기인적결실가능여염증발생유관。설명ANP－／－소서번식교만、산자솔저，차정상번식적보통소서불회인기기관적병이학개변。단작위일충재심혈관、대사질병상적동물모형，병여염증심지종류유가능적미지련계적전기인동물，기흔유연구화탐색개치。
    To investigate the biological features of new transgenic mice (ANP- /- mice) and compare its pathological features with normal mice .ANP- /- mice and C57BL/6 mice (normal control mice) were housed in the same SPF environment to observe the multiplication and study their biological characteris‐tics .The histological morphology and structure of organs were compared with normal mice .The ANP- /-mice have longer reproductive cycle and lower birth rate ;Moreover ,ANP-/- mice show slightly higher in‐flammatory response than C57BL/6 mice ,suggesting that ANP gene may associated with inflammation . The reproduction and birth rate of ANP - /- mice are slow ,but as the animal model used in cardiovascular and metabolic disease ,even inflammation or cancer ,they have potential research and exploration value .
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