CAJ | 학술논문

为了解云南省犬细小病毒V P2基因分子流行变异情况，对来源于云南省7个地级市13个犬细小病毒样品进行了V P2基因的扩增和序列分析，并与相关参考序列进行了遗传进化比对分析。结果表明，13个样品中9个基因型为CPV‐2a ，3个基因型为CPV‐2b ，1个基因型为CPV‐2。氨基酸序列变异主要集中在297位～518位氨基酸处，其中324位和440位氨基酸变异在犬细小病毒进化过程中扮演着重要角色。遗传进化分析显示10个样品序列形成了一个相对于国内外参考序列独立的遗传进化分支，提示云南省犬细小病毒以CPV‐2a基因型流行为主。
위료해운남성견세소병독V P2기인분자류행변이정황，대래원우운남성7개지급시13개견세소병독양품진행료V P2기인적확증화서렬분석，병여상관삼고서렬진행료유전진화비대분석。결과표명，13개양품중9개기인형위CPV‐2a ，3개기인형위CPV‐2b ，1개기인형위CPV‐2。안기산서렬변이주요집중재297위～518위안기산처，기중324위화440위안기산변이재견세소병독진화과정중분연착중요각색。유전진화분석현시10개양품서렬형성료일개상대우국내외삼고서렬독립적유전진화분지，제시운남성견세소병독이CPV‐2a기인형류행위주。
In order to investigate the prevalence and variation of VP 2 gene of CPV in Yunnan province ,the gene amplification and sequence analysis were performed for thirteen strains of canine parvovirus ,compri‐sing of 9 strains of CPV‐2a ,3 strains of CPV‐2b and 1 strain of CPV‐2 .It was shown that most of the a‐mino acid variations occurred within the region of position 297‐518 .A high level of substitution at 324 and 440 sites was associated with the evolution of antigenic variants in circulating parvovirus types .Based on the phylogenetic analysis on the amino acid level of the VP2 proteins ,the thirteen strains of CPVs were grouped into 3 clusters .Nine of them belonged to CPV‐2a ,three belonged to CPV‐2b ,and one belonged to CPV‐2 .The result showed that CPV‐2a was the main prevalent strain in Yunnan province and formed the independent branch .