CAJ | 학술논문

运用RT‐PCR的方法，对猪瘟病毒E0基因进行扩增、克隆及测序，运用DNA Star分析软件对6株毒株与猪瘟兔化弱毒苗（HCLV）、石门（Shimen）株、Paderborn株、GXWZ02株的相应片段进行比较分析，构建了CSFV遗传进化树。序列分析表明，广西流行株与HCLV株、Shimen株、Paderborn株、GXWZ02株之间核苷酸的同源性分别为80．8％～81．5％、82．8％～83．3％、93．1％～94．2％、93．7％～94．2％，推导的氨基酸同源性分别为88．3％～89．7％、89．7％～91．1％、96．2％～97．7％、97．7％～99．1％；6株广西C S‐FV流行毒株与国内外已发表的14株病毒相应序列进行比较，构建遗传进化树，结果表明所比较的20株毒株分为2个基因群，广西流行毒株均属于基因群Ⅱ。本研究成功地对广西流行猪瘟病毒株的E0基因进行了测序分析，表明近年来广西流行毒株未发生较大的变异，但病毒株有远离疫苗株发展的趋势。
운용RT‐PCR적방법，대저온병독E0기인진행확증、극륭급측서，운용DNA Star분석연건대6주독주여저온토화약독묘（HCLV）、석문（Shimen）주、Paderborn주、GXWZ02주적상응편단진행비교분석，구건료CSFV유전진화수。서렬분석표명，엄서류행주여HCLV주、Shimen주、Paderborn주、GXWZ02주지간핵감산적동원성분별위80．8％～81．5％、82．8％～83．3％、93．1％～94．2％、93．7％～94．2％，추도적안기산동원성분별위88．3％～89．7％、89．7％～91．1％、96．2％～97．7％、97．7％～99．1％；6주엄서C S‐FV류행독주여국내외이발표적14주병독상응서렬진행비교，구건유전진화수，결과표명소비교적20주독주분위2개기인군，엄서류행독주균속우기인군Ⅱ。본연구성공지대엄서류행저온병독주적E0기인진행료측서분석，표명근년래엄서류행독주미발생교대적변이，단병독주유원리역묘주발전적추세。
Using RT‐PCR method ,E0 gene of six CSFV strains were amplified ,cloned and sequenced . Then the sequences were analyzed and compared with the same gene sequences of HCLV strain ,Shimen strain ,Paderborn strain and GXWZ02 strain by DNAstar software . The nucleotide homologies were 80 .8%‐81 .5% ,82 .8%‐83 .3% ,93 .1%‐94 .2% and 93 .7%‐94 .2% , respectively , and amino acid w ere 88 .3%‐89 .7% ,89 .7%‐91 .1% ,96 .2%‐97 .7% and 97 .7%‐99 .1% with reference stains .The phylogenet‐ic analysis showed that GX strains and other 14 strains were classified as 2 groups ,while all the GX strains belonged to genotype Ⅱ .The study successfully sequenced the E0 gene of six CSFV strains in Guangxi . The results revealed that there is no large variation in Guangxi pandemic strains in recent years ,but a trend that virus development is far away from vaccine strain .