CAJ | 학술논문

将检测为犬细小病毒（CPV ）阳性的病料经处理后接种F81细胞盲传3代并获得一株细小病毒，命名为JL‐M13。运用重叠PCR技术从JL‐M13中扩增出5条核苷酸序列，拼接后进行序列分析。结果表明，所获病毒核酸基因组大小为4621 bp ，与其他犬细小病毒的基因相似性为98．2％～99．4％。系统发育树分析表明，JL‐M13与CPV‐2毒株（EF011664．1）相似率最高，与 Type‐2型犬细小病毒疫苗株相距较远。
장검측위견세소병독（CPV ）양성적병료경처리후접충F81세포맹전3대병획득일주세소병독，명명위JL‐M13。운용중첩PCR기술종JL‐M13중확증출5조핵감산서렬，병접후진행서렬분석。결과표명，소획병독핵산기인조대소위4621 bp ，여기타견세소병독적기인상사성위98．2％～99．4％。계통발육수분석표명，JL‐M13여CPV‐2독주（EF011664．1）상사솔최고，여 Type‐2형견세소병독역묘주상거교원。
We inoculated canine parvovirus positive samples into F 81 cells and isolated a virus strain named JL‐M13 by three passages .Five nucleotide sequences were amplified from the DNA extracted from canine parvovirus wild strain by overlapping PCR .Splicing and analysis of the sequences showed that the genome length of the strain were 4621bp ,the identity with other canine parvovirus was 98 .2%‐99 .4% .Phyloge‐netic tree analysis showed that the similarity rate of JL‐M13 with CPV‐2 (EF011664 .1)was the highest and was significantly lower with canine parvovirus type‐2 vaccine strain .