CAJ | 학술논문

草鱼呼肠孤病毒（Grass carp reovirus, GCRV）是草鱼出血病的病原。从患病草鱼体内分离到一株草鱼呼肠孤病毒GCRV 096，经反转录PCR，克隆得GCRV 096长度为855 bp的vp7基因，并分析该基因编码蛋白的特性。结果表明，同一基因型分离株 VP7蛋白间的差异不大，但不同基因型分离株 VP7蛋白间存在很大的差异。对GCRV 096 VP7蛋白生物信息学分析表明，信号肽位点位于20氨基酸处，且VP7含有4个潜在的抗原决定簇。构建GCRV 096vp7基因的酵母表达载体pGBKT7-S10，并成功转化至酵母中。
초어호장고병독（Grass carp reovirus, GCRV）시초어출혈병적병원。종환병초어체내분리도일주초어호장고병독GCRV 096，경반전록PCR，극륭득GCRV 096장도위855 bp적vp7기인，병분석해기인편마단백적특성。결과표명，동일기인형분리주 VP7단백간적차이불대，단불동기인형분리주 VP7단백간존재흔대적차이。대GCRV 096 VP7단백생물신식학분석표명，신호태위점위우20안기산처，차VP7함유4개잠재적항원결정족。구건GCRV 096vp7기인적효모표체재체pGBKT7-S10，병성공전화지효모중。
Grass Carp Reovirus (GCRV) is the aetiological agent of grass carp hemorrhage. GCRV 096 was isolated from the diseased grass carp. After RT-PCR, thevp7 gene in GCRV 096, 855 bp in length, was cloned. Characteristics of the VP7 protein, encoded by thevp7 gene, were analyzed. The results showed that there was very little difference of the VP7 proteins in GCRV strains of the same genotype, but there was much difference in that of GCRV strains of different genotypes. Bioinformatics analysis of the VP7 protein indicated that the signal peptide site was located at 20 amino acid, and the VP7 protein had four potential antigenic determinants. Furthermore, yeast expression vector pGBKT7-S10 of thevp7gene was constructed, and successfully transformed into yeast cells.