CAJ | 학술논문

双组分调控系统（Two-component Regulatory System）在致病菌的生长及毒力调控中起重要作用。克隆溶藻弧菌（Vibrio alginolyticus）HY9901株组氨酸激酶PhoR和反应调控因子PhoB的全长基因，并对其进行生物信息学分析。序列分析结果显示，phoR（GenBank登录号：KJ958404）全长1299 bp，共编码432个氨基酸；phoB （GenBank登录号：KJ863646）全长690 bp，编码229个氨基酸。构建PhoR/PhoB的系统进化树，结果显示，溶藻弧菌PhoR/PhoB与副溶血弧菌（Vibrio parahaemolyticus）、哈氏弧菌（Vibrio harveyi）有较近亲缘关系。利用SWISS-MODEL软件，对PhoR/PhoB中两个相对保守的功能域HATPase_c和REC进行同源建模，发现HATPase_c具有1个ATP结合位点，REC具有4个天冬氨酸活性位点。
쌍조분조공계통（Two-component Regulatory System）재치병균적생장급독력조공중기중요작용。극륭용조호균（Vibrio alginolyticus）HY9901주조안산격매PhoR화반응조공인자PhoB적전장기인，병대기진행생물신식학분석。서렬분석결과현시，phoR（GenBank등록호：KJ958404）전장1299 bp，공편마432개안기산；phoB （GenBank등록호：KJ863646）전장690 bp，편마229개안기산。구건PhoR/PhoB적계통진화수，결과현시，용조호균PhoR/PhoB여부용혈호균（Vibrio parahaemolyticus）、합씨호균（Vibrio harveyi）유교근친연관계。이용SWISS-MODEL연건，대PhoR/PhoB중량개상대보수적공능역HATPase_c화REC진행동원건모，발현HATPase_c구유1개ATP결합위점，REC구유4개천동안산활성위점。
Two-component regulatory system plays an important role in the growth and virulence of bacteria. In this study, the phosphate regulatory sensor protein (PhoR) gene and phosphate transcriptional regulatory protein(PhoB) gene ofVibrio alginolyticus strain HY9901 were cloned. Sequence analysis revealed that the length ofphoR gene was 1 299 bp, encoding a putative protein of 432 amino acids. The length ofphoB gene was 690 bp and encodes a putative protein of 299 amino acids. A phylogenetic tree was constructed by MEGA 5.0 software, indicating that the PhoR and PhoB ofV. alginolyticus showed high genetic relationship withVibrioparahaemolyticusand Vibrio harveyi. By SWISS-MODEL work-space, the three-dimensional structures of HATPase_c and REC conserved domain in the PhoR and PhoB were determined, showing that HATPase_c and REC domain contained an ATP binding site and four aspartic acid active sites, respectively, playing critical roles in regulation of growing and virulence. These results would provide a basis for the further studies on the biological function and drug development of PhoR / PhoB.