中国临床药理学杂志
中國臨床藥理學雜誌
중국림상약이학잡지
THE CHINESE JOURNAL OF CLINICAL PHARMACOLOGY
2015年
1期
63-66
,共4页
徐划萍%金国琴%龚张斌%康湘萍
徐劃萍%金國琴%龔張斌%康湘萍
서화평%금국금%공장빈%강상평
补肾益气方药%衰老%半乳糖%骨骼肌%抗氧化能力
補腎益氣方藥%衰老%半乳糖%骨骼肌%抗氧化能力
보신익기방약%쇠로%반유당%골격기%항양화능력
Bushen Yiqi%aging%D-galactose%skeletal muscle%antioxidant capacity
目的：观察补肾益气方药对衰老大鼠运动能力、骨骼肌形态及抗氧化能力等的影响。方法 SD雄性大鼠分为正常组、模型组、中药组及美雄酮组。后3组大鼠于颈背部皮下注射10％D－半乳糖0．1 mg? g －1? d－1，中药及美雄酮组同时分别灌服药物，用药剂量分别为临床成人每千克体重的7倍及等效剂量，连续10周，造模结束。跑台测试大鼠运动能力；电镜观察骨骼肌形态结构；Elisa法测定高级糖化终末产物（ AGEs）；分光光度法测试血清超氧化物歧化酶（ SOD）、丙二醛（ MDA）含量。结果与正常组相比，模型组运动能力及骨骼肌线粒体数量、体积、SOD活性、胸腺指数和脾指数均明显下降，而血清AGEs、MDA含量则显著增高。中药和美雄酮组较模型组运动能力、骨骼肌线粒体数量、体积以及胸腺指数显著升高， MDA明显下降。结论补肾益气方药可通过改善模型大鼠上述各项指标的退行性变化，延缓机体衰老。
目的：觀察補腎益氣方藥對衰老大鼠運動能力、骨骼肌形態及抗氧化能力等的影響。方法 SD雄性大鼠分為正常組、模型組、中藥組及美雄酮組。後3組大鼠于頸揹部皮下註射10％D－半乳糖0．1 mg? g －1? d－1，中藥及美雄酮組同時分彆灌服藥物，用藥劑量分彆為臨床成人每韆剋體重的7倍及等效劑量，連續10週，造模結束。跑檯測試大鼠運動能力；電鏡觀察骨骼肌形態結構；Elisa法測定高級糖化終末產物（ AGEs）；分光光度法測試血清超氧化物歧化酶（ SOD）、丙二醛（ MDA）含量。結果與正常組相比，模型組運動能力及骨骼肌線粒體數量、體積、SOD活性、胸腺指數和脾指數均明顯下降，而血清AGEs、MDA含量則顯著增高。中藥和美雄酮組較模型組運動能力、骨骼肌線粒體數量、體積以及胸腺指數顯著升高， MDA明顯下降。結論補腎益氣方藥可通過改善模型大鼠上述各項指標的退行性變化，延緩機體衰老。
목적：관찰보신익기방약대쇠로대서운동능력、골격기형태급항양화능력등적영향。방법 SD웅성대서분위정상조、모형조、중약조급미웅동조。후3조대서우경배부피하주사10％D－반유당0．1 mg? g －1? d－1，중약급미웅동조동시분별관복약물，용약제량분별위림상성인매천극체중적7배급등효제량，련속10주，조모결속。포태측시대서운동능력；전경관찰골격기형태결구；Elisa법측정고급당화종말산물（ AGEs）；분광광도법측시혈청초양화물기화매（ SOD）、병이철（ MDA）함량。결과여정상조상비，모형조운동능력급골격기선립체수량、체적、SOD활성、흉선지수화비지수균명현하강，이혈청AGEs、MDA함량칙현저증고。중약화미웅동조교모형조운동능력、골격기선립체수량、체적이급흉선지수현저승고， MDA명현하강。결론보신익기방약가통과개선모형대서상술각항지표적퇴행성변화，연완궤체쇠로。
Objective To observe the effects of Bushen Yiqi recipe on aging rats′ exercise capacity, morphology and oxidation resis-tance.Methods Male sprague dawley rats were divided into normal group, model group, traditional Chinese medicine group, methandienone group.The rats in the latter three group were received a subcutaneous in-jection of 10%D-galactose with 0.1 mg? g -1? d-1 for 10 weeks.The rats in traditional Chinese medicine group and methandienone group were given medicine by gavage .The dosage is adults′7 times of per kilogram of body weight and equivalent dose respectively.The experiment were las-ted for 10 weeks.Then rats′exercise capacity were tested by treadmill. The Morphological structure of the skeletal muscle were observed by elec-tron microscope.Rats′advanced glycosylated end products( AGEs) were tested by Elisa method.Rats′superoxidedismutase ( SOD) and malondi-aldehyde ( MDA) content in blood serum were tested by spectrophotome-try.Results Compared with normal group, the rats′exercise capacity, the mitochondria number and volume of rats′skeletal muscle, the serum SOD activity, the thymus index and spleen index in model group dropped significantly.The serum AGEs and MDA content increased significant-ly.Compared with model group, the exercise capacity, the mitochondria number and volume of rats′skeletal muscle, the thymus index in traditional Chinese medicine group and methandienone group inceased obviously.The serum MDA content decreased obviously.Conclusion Bushen Yiqi recipe can slow the aging progress of skeletal muscle through improving the model rats′degenerative changes of the above-mentioned indexes.
