CAJ | 학술논문

目的：研究四逆散冻干粉主要化学成分芍药苷改善睡眠的作用机制。方法取Wistar大鼠平分为4组（每组10只）：正常组、四逆散冻干粉组、白芍冻干粉组、芍药苷组。称取冻干粉20 g，加水溶解配成0．5 kg? L－1溶液，灌胃给药，正常组给予等体积蒸馏水。每天1次，连续7 d。用HPLC方法测定大鼠血清和脑脊液中移行成分。结果给予四逆散冻干粉主要化学成分芍药苷后，脑脊液中内源性物质峰面积约是空白脑脊液峰面积的12．5倍，表明芍药苷能明显促进脑脊液中内源性物质的分泌，从而达到改善睡眠作用，该内源性物质最终确定为5－羟色胺。结论四逆散冻干粉主要化学成分芍药苷是通过促进脑脊液中内源性物质5－羟色胺的分泌达到改善睡眠作用。
목적：연구사역산동간분주요화학성분작약감개선수면적작용궤제。방법취Wistar대서평분위4조（매조10지）：정상조、사역산동간분조、백작동간분조、작약감조。칭취동간분20 g，가수용해배성0．5 kg? L－1용액，관위급약，정상조급여등체적증류수。매천1차，련속7 d。용HPLC방법측정대서혈청화뇌척액중이행성분。결과급여사역산동간분주요화학성분작약감후，뇌척액중내원성물질봉면적약시공백뇌척액봉면적적12．5배，표명작약감능명현촉진뇌척액중내원성물질적분비，종이체도개선수면작용，해내원성물질최종학정위5－간색알。결론사역산동간분주요화학성분작약감시통과촉진뇌척액중내원성물질5－간색알적분비체도개선수면작용。
Objective To study on sleep improving mechanism of Si Ni San Freeze -dry Powder.Methods Wistar rats were divided into four groups, each group was 10.Which were normal group, Si Ni San powder group, Radix Paeoniae Alba group.Paeoniflorin group.The freeze-dried powder 20 g, water dissolved into 0.5 kg? L-1 solution, administered orally, the normal group were given equal volume of distilled water,1 times a day, continuous 7 d.Blood transitional component were analzed by HPLC method.Results By the analysis of the compound in cerebrospinal fluid, the endogenous substance in cerebrospinal fluid was increased obvi-ously.Increasing peak area of the endogenous substance in cerebrospinal fluid were about 12.5 times the blank′s in cerebrospinal fluid.Conclusion Paeoniflorin in Si Ni San Freeze-dry Powder improves sleep by promo-ting the secretion of 5-serotonin in cerebrospinal fluid.