CAJ | 학술논문

目的：探讨制何首乌对链脲佐菌素（ STZ）诱导的糖尿病大鼠降血糖作用及可能机制。方法用STZ（50 mg? kg－1）腹腔注射建立1型糖尿病（ T1DM）大鼠模型；建模第4周随机分为 T1 DM 模型组和何首乌低、高剂量组（1，2 g? kg－1? d－1），另取正常大鼠做对照组，对照组和模型组灌胃等量的0．9％NaCl，直至第12周。分别于第5，12周检测大鼠体重、血糖；对胰腺组织进行HE染色，用免疫组织化学法检测β细胞胰岛素的含量，用末端脱氧核苷酸转移酶标记法（ TUNEL）检测胰岛细胞凋亡。结果与对照组相比，模型组大鼠有明显的“三多一少”症状；与模型组相比，何首乌高剂量组体重明显增加，血糖明显降低，胰岛细胞凋亡指数明显下降，胰岛素表达阳性率明显升高（均 P ＜0．05）；但低剂量组上述指标改变差异不显著。结论制何首乌降低T1DM大鼠血糖机制与提高胰岛素表达、抑制胰岛细胞凋亡有关。
목적：탐토제하수오대련뇨좌균소（ STZ）유도적당뇨병대서강혈당작용급가능궤제。방법용STZ（50 mg? kg－1）복강주사건립1형당뇨병（ T1DM）대서모형；건모제4주수궤분위 T1 DM 모형조화하수오저、고제량조（1，2 g? kg－1? d－1），령취정상대서주대조조，대조조화모형조관위등량적0．9％NaCl，직지제12주。분별우제5，12주검측대서체중、혈당；대이선조직진행HE염색，용면역조직화학법검측β세포이도소적함량，용말단탈양핵감산전이매표기법（ TUNEL）검측이도세포조망。결과여대조조상비，모형조대서유명현적“삼다일소”증상；여모형조상비，하수오고제량조체중명현증가，혈당명현강저，이도세포조망지수명현하강，이도소표체양성솔명현승고（균 P ＜0．05）；단저제량조상술지표개변차이불현저。결론제하수오강저T1DM대서혈당궤제여제고이도소표체、억제이도세포조망유관。
Objective To discuss the effect on Polygonum lowering streptozotocin ( STZ) rats′blood glucose and explore its possible mecha-nism.Methods The rats of type 1 of diabets( T1DM) was induced by 50 mg? kg-1 STZ.Those rats were randomly divided into T1DM group, low-dose and high-dose group of Polygonum(1,2 g? kg -1? d -1 ), another nomal rats as the control group.T1 DM group and control group were gavaged equal 0.9%NaCl from the fourth week to the 12 nd week. The rats′weight and blood glucose would be measured in the fifth week and the twelve week. Then the pancreas tissue were made HE stai-ning.The level of insulin in the beta cells was detected by immunohisto-chemical staining,and apoptosis of islet cells was marked by terminal de-oxynucleotidyl transferase mediated dUTP biotin nick end labeling (TUNEL) .Results Compared with the control group , T1DM rats showed symptoms of polydipsia, polyuria, polyphagia, weight loss, high level of blood sugar and insulin structural damage.Compared with the T1DM group, high -dose group of Polygonum were generally better:weight was increasd ( P <0.05 ) , blood glucose was lower ( P <0.05 ) , while islet cells apoptosis index decreased significantly( P<0.05) ,insu-lin-positive expression was improved ( P <0.05 ) .Those indicators of low-dose group had no significant difference.Conclusion Polygonum can reduce the STZ rats′blood glucose probably by improving the expression of insulin and inhibiting the apoptosis of islet cells.