中国临床药理学杂志
中國臨床藥理學雜誌
중국림상약이학잡지
THE CHINESE JOURNAL OF CLINICAL PHARMACOLOGY
2015年
1期
31-33,37
,共4页
雷公藤内酯醇%糖尿病肾脏疾病%节活化正常T细胞表达与分泌因子
雷公籐內酯醇%糖尿病腎髒疾病%節活化正常T細胞錶達與分泌因子
뢰공등내지순%당뇨병신장질병%절활화정상T세포표체여분비인자
triptolide%diabetic kidney disease%regulated upon activation normal T-cell expressed and secreted
目的：研究糖尿病大鼠肾皮质RANTES和desmin表达及雷公藤内酯醇对其干预作用。方法 SD大鼠随机分为正常组（ NG）、模型组（ DM）和小、大剂量雷公藤内酯醇组（T1，T2：0．2，0．4 mg? kg－1），灌胃。实验第4，8，12周，检测各组5只大鼠的血糖、肾重、24 h尿白蛋白；同时观察肾皮质中RANTES和demin mRNA的表达。结果 DM组，肾皮质RANTES和demin的表达在第4，8，12周较NG组显著升高（ P ＜0．05），且从第4周末起，逐渐增高到12周末（ P ＜0．05）；T1、T2组较DM组表达量显著降低（ P ＜0．01）；且T2组较T1组各周的表达更低（ P＜0．05）；从第4周末起，T1组的RANTES mRNA表达逐渐下降至12周末（ P＜0．05）；与第4周末相比，T2组的RANTES mRNA表达在第12周末显著下降（ P＜0．01）；T1组和T2组的desmin mRNA表达在第12周末均较第4周末均显著下降（ P＜0．05）。 DM组RANTES mRNA的表达与desmin mRNA的表达和24 h尿蛋白显著正相关。结论雷公藤内酯醇可能通过抑制糖尿病大鼠肾皮质RANTES的过度表达，缓解足细胞损伤，减轻蛋白尿。
目的：研究糖尿病大鼠腎皮質RANTES和desmin錶達及雷公籐內酯醇對其榦預作用。方法 SD大鼠隨機分為正常組（ NG）、模型組（ DM）和小、大劑量雷公籐內酯醇組（T1，T2：0．2，0．4 mg? kg－1），灌胃。實驗第4，8，12週，檢測各組5隻大鼠的血糖、腎重、24 h尿白蛋白；同時觀察腎皮質中RANTES和demin mRNA的錶達。結果 DM組，腎皮質RANTES和demin的錶達在第4，8，12週較NG組顯著升高（ P ＜0．05），且從第4週末起，逐漸增高到12週末（ P ＜0．05）；T1、T2組較DM組錶達量顯著降低（ P ＜0．01）；且T2組較T1組各週的錶達更低（ P＜0．05）；從第4週末起，T1組的RANTES mRNA錶達逐漸下降至12週末（ P＜0．05）；與第4週末相比，T2組的RANTES mRNA錶達在第12週末顯著下降（ P＜0．01）；T1組和T2組的desmin mRNA錶達在第12週末均較第4週末均顯著下降（ P＜0．05）。 DM組RANTES mRNA的錶達與desmin mRNA的錶達和24 h尿蛋白顯著正相關。結論雷公籐內酯醇可能通過抑製糖尿病大鼠腎皮質RANTES的過度錶達，緩解足細胞損傷，減輕蛋白尿。
목적：연구당뇨병대서신피질RANTES화desmin표체급뢰공등내지순대기간예작용。방법 SD대서수궤분위정상조（ NG）、모형조（ DM）화소、대제량뢰공등내지순조（T1，T2：0．2，0．4 mg? kg－1），관위。실험제4，8，12주，검측각조5지대서적혈당、신중、24 h뇨백단백；동시관찰신피질중RANTES화demin mRNA적표체。결과 DM조，신피질RANTES화demin적표체재제4，8，12주교NG조현저승고（ P ＜0．05），차종제4주말기，축점증고도12주말（ P ＜0．05）；T1、T2조교DM조표체량현저강저（ P ＜0．01）；차T2조교T1조각주적표체경저（ P＜0．05）；종제4주말기，T1조적RANTES mRNA표체축점하강지12주말（ P＜0．05）；여제4주말상비，T2조적RANTES mRNA표체재제12주말현저하강（ P＜0．01）；T1조화T2조적desmin mRNA표체재제12주말균교제4주말균현저하강（ P＜0．05）。 DM조RANTES mRNA적표체여desmin mRNA적표체화24 h뇨단백현저정상관。결론뢰공등내지순가능통과억제당뇨병대서신피질RANTES적과도표체，완해족세포손상，감경단백뇨。
Objective To evaluate the effects of triptolide on the renal tissue of diabetic rats and its possible mechanisms.Methods SD rats were randomly divided into normal group ( NG ) , diabetic model group (DM), low and high doses triptolide treatment groups( T1, T2, dose:0.2 , 0.4 mg? kg -1 ) .Five rats of each group were randomly selected and the organ specimens were removed at the end of the 4th, 8th and 12th week of the experiment. Kidney weight, 24 hours urinry albumin (24hUAL) , plasma glucose were determined.The mRNA expression of regulated upon activation normal T -cell expressed and secreted ( RANTES) and desmin in kidney tissue was assessed by RT-PCR.Re-sults Compared with the NG group, the expressions of RANTES and desmin mRNA in the DM group were significantly increased.Further-more, they were significantly increased from the end of 4th week through the end of 12th week within DM groups.Compared with the DM group, the expressions of RANTES and desmin mRNA in the T1 and T2 group were significantly decreased.Furthermore, they were significantly de-creased in T2 group in comparison with those in T1 group at the end of observational weeks. The expression of RANTES mRNA significantly decreased from the end of 4th week through the end of 12th week within T1 groups.Compared with that at the end of 4th week, the expression of it was significantly decreased at the end of 12th week within the T2 groups.Compared with that at the end of 4th week, the expression of desmin mRNA was significantly decreased at the end of 12th week within the both of T1 and T2 groups.The expression of RANTES positively correlated to desmin and 24 h proteinuria in the renal cortex of diabetic rats before the triptolide intervention.Conclusion Triptolide could significantly alleviate podocyte depletion and attenuate the diabetic kidney disease associated proteinuria via significantly inhabiting the overexpression of RANTES in the renal cortex of diabetic rats.
