CAJ | 학술논문

目的：观察固本咳喘颗粒（ GKG）对慢性阻塞性肺疾病（ COPD）大鼠可溶性白细胞介素－2受体（sIL－2R）表达的影响。方法用烟熏加气管内滴注脂多糖建立COPD大鼠模型，随机分为6组：正常组、模型组、3个剂量GKG组及固本咳喘片组。从造模的第3天开始，正常组和模型组灌服0．9％氯化钠10 mL? kg－1，GKG组分别灌服3个剂量GKG混悬液（2．28，1．14，0．57 g? mL－1），固本咳喘片组灌服混悬液0．01 g? mL－1，灌胃1次／日，共28 d。灌胃结束第2天处死大鼠，留取标本。用双抗体夹心ABC－ELISA法检测大鼠sIL－2R。结果小剂量GKG组和固本咳喘片组能降低sIL－2R表达，与模型组比较差异有统计学意义（P＜0．05）。结论 GKG可通过调控sIL－2R，从而提高COPD大鼠免疫功能及抗感染能力。
목적：관찰고본해천과립（ GKG）대만성조새성폐질병（ COPD）대서가용성백세포개소－2수체（sIL－2R）표체적영향。방법용연훈가기관내적주지다당건립COPD대서모형，수궤분위6조：정상조、모형조、3개제량GKG조급고본해천편조。종조모적제3천개시，정상조화모형조관복0．9％록화납10 mL? kg－1，GKG조분별관복3개제량GKG혼현액（2．28，1．14，0．57 g? mL－1），고본해천편조관복혼현액0．01 g? mL－1，관위1차／일，공28 d。관위결속제2천처사대서，류취표본。용쌍항체협심ABC－ELISA법검측대서sIL－2R。결과소제량GKG조화고본해천편조능강저sIL－2R표체，여모형조비교차이유통계학의의（P＜0．05）。결론 GKG가통과조공sIL－2R，종이제고COPD대서면역공능급항감염능력。
Objective To observe the effect of Guben Kechuan Gran-ules ( GKG ) on expression level of soluble interleukin -2 receptor (sIL-2R) of rats with chronic obstructive pulmonary disease (COPD). Methods Rat models with COPD made by fumigation and dripping li-popolysaccharide.The rtas were randomly divided into 6 groups:normal group, model group, high, middle, low doses ( 2.28, 1.14, 0.57 g? mL-1 ) GKG groups, and Guben Kechuan Tablets group.From the third day of modeling, medicines were administered to rats by means of intragastric administration for consecutive 28 days.Two days after the fin-ishing of intragastric administration,rats were executed and samples were collected.SIL-2 R expression level in rat was tested by means of ABC-ELISA method.Results SIL-2R expression level in low-dose groups of GKG decreased obviously,with a significant difference compared with model group ( P<0.05).Conclusion GKG can enhance immune func-tion and anti infection ability in the airway of rats with experimental COPD by regulating sIL-2R expression level.