CAJ | 학술논문

目的：体外诱导近平滑假丝酵母菌（ CP）、热带假丝酵母菌（ CT）对两性霉素B（ AMB）的耐药株，检测其中的耐药基因表达情况。方法 AMB体外构建耐药株，用实时荧光定量PCR法检测耐药基因的表达水平。结果 CP、CT 野生株经体外 AMB 浓度递增法诱导后，形成耐药株最低抑菌浓度（ MIC，≥8μg? mL－1）；CYP51A、CYP51B、CDR1、CDR2、MDR1及MDR2基因在野生株中低水平表达，在耐药株中表达水平明显升高，差别有统计学意义（ P ＜0．05）；CYP51A、CYP51B、CDR1、CDR2、MDR1及MDR2的表达量在CPAMB耐药株中分别是其野生株的136．92，16．33，2．07，14．84，4．07，2．38倍；在CT AMB耐药株中分别是其野生株的8．83，2．98，3．00，2．40，2．30，3．57倍。结论 CP、CT对AMB耐药与CYP51A、CYP51B、CDR1、CDR2、MDR1及MDR2基因过度表达有关。
목적：체외유도근평활가사효모균（ CP）、열대가사효모균（ CT）대량성매소B（ AMB）적내약주，검측기중적내약기인표체정황。방법 AMB체외구건내약주，용실시형광정량PCR법검측내약기인적표체수평。결과 CP、CT 야생주경체외 AMB 농도체증법유도후，형성내약주최저억균농도（ MIC，≥8μg? mL－1）；CYP51A、CYP51B、CDR1、CDR2、MDR1급MDR2기인재야생주중저수평표체，재내약주중표체수평명현승고，차별유통계학의의（ P ＜0．05）；CYP51A、CYP51B、CDR1、CDR2、MDR1급MDR2적표체량재CPAMB내약주중분별시기야생주적136．92，16．33，2．07，14．84，4．07，2．38배；재CT AMB내약주중분별시기야생주적8．83，2．98，3．00，2．40，2．30，3．57배。결론 CP、CT대AMB내약여CYP51A、CYP51B、CDR1、CDR2、MDR1급MDR2기인과도표체유관。
Objective To induce the amphoterincin B(AMB) resistant strains of the Candida parapsilosis( CP) and the Candida tropicalis( CT) in vitro, to test the expressions of the drug-resistant genes and discuss the corresponding molecular mechanisms.Methods The resistant strains were induced with amphoterincin B ( AMB) in vitro .The drug-resis-tant gene expression levels of the wild strains and the resistant strains were measured by real -time fluorescence quantitative PCR ( qPCR ) method.Results The wild strains of the CP and CT were induced to the resistant strains minimal inhibitory concentration( MIC,≥8 μg? mL-1 ) with AMB in vitro.The wild strains have CYP51A, CYP51B, CDR1, CDR2 , MDR1 , MDR2 mRNA expression at low level in the CP and CT.But the mRNA expression level of the CYP51A, CYP51B, CDR1, CDR2 , MDR1 , MDR2 was higher in the AMB resistant strains than the wild strains ( P <0.05 ) .The expression multiple of the CYP51A, CYP51B, CDR1, CDR2, MDR1, MDR2 were 136.92, 16.33, 2.07, 14.84, 4.07, 2.38 times in the CP AMB resistant strains compared to the wild strains, respectively.But they were 8.83, 2.98, 3.00, 2.40, 2.30 ,3.57 times respectively in the CT AMB resistant strains compared to the wild strains.Conclusion The excessive expression of the CYP51A,CYP51B,CDR1,CDR2,MDR1, MDR2 mRNA were associated with the resistance to AMB in the CP and CT strains.