CAJ | 학술논문

目的：了解南昌四家教学医院的碳青霉烯类耐药肺炎克雷伯菌（ CRKP）分子流行病学特征。方法收集2011年1月至2012年6月来自南昌地区四家教学医院临床分离的29株亚胺培南和／或美罗培南耐药肺炎克雷伯菌，聚合酶链反应（PCR）检测CRKP携带的耐药基因；用多位点序列分型技术（MLST）对CRKP进行分子遗传学分析；eBURST软件对多位点序列分型结果进行其种群结构分析。结果29株CRKP经MLST成功分成16个ST（序列类型）型。其中，以ST11和ST15为主（各5株）；其次为ST395（3株），ST334、ST273、ST147（各2株），ST1128、ST42、ST1031、ST764、ST629和ST37（各1株）；值得注意的是，4个新的ST型已被PubMlst数据库确认收录并分别命名为ST1369、ST1411、ST1412和ST1413。其中，有2个 ST 群，每个 ST 群分别包括2个 ST 型（ ST395和ST1369，ST147和ST273），其他12个ST型都是单个的ST型，其中ST11、ST15和ST395均属于CC258克隆复合体。结论本地区CRKP具有基因多态性，主要克隆系为ST11和ST15，曾有过短暂局部的ST15流行。
목적：료해남창사가교학의원적탄청매희류내약폐염극뢰백균（ CRKP）분자류행병학특정。방법수집2011년1월지2012년6월래자남창지구사가교학의원림상분리적29주아알배남화／혹미라배남내약폐염극뢰백균，취합매련반응（PCR）검측CRKP휴대적내약기인；용다위점서렬분형기술（MLST）대CRKP진행분자유전학분석；eBURST연건대다위점서렬분형결과진행기충군결구분석。결과29주CRKP경MLST성공분성16개ST（서렬류형）형。기중，이ST11화ST15위주（각5주）；기차위ST395（3주），ST334、ST273、ST147（각2주），ST1128、ST42、ST1031、ST764、ST629화ST37（각1주）；치득주의적시，4개신적ST형이피PubMlst수거고학인수록병분별명명위ST1369、ST1411、ST1412화ST1413。기중，유2개 ST 군，매개 ST 군분별포괄2개 ST 형（ ST395화ST1369，ST147화ST273），기타12개ST형도시단개적ST형，기중ST11、ST15화ST395균속우CC258극륭복합체。결론본지구CRKP구유기인다태성，주요극륭계위ST11화ST15，증유과단잠국부적ST15류행。
Objective To investigate the molecular epidemiology of car-bapenem resistant Klebsiella pneumonia( CRKP) from four teaching hos-pitals in Nanchang by multilocus sequence typing ( MLST ) .Methods Twenty-nine imipenem and/or meropenem resistant Klebsiella pneumoni-a isolates were totally collected from four teaching hospitals in Nanchang from Jan 2011 to June 2012.The polymerase chain reaction( PCR) am-plifications of drug resistant genes were performed.The molecular genetic of CRKPs were analyzed by MLST.The population structure of CRKPs was evaluated by eBURST with the results of MLST.Results Twenty-nine CRKPs were successfully divided into 16 sequence types ( STs ) by MLST.The main types were ST11 and ST15 (5/29 each); followed by ST395 ( 3/29 ); ST334, ST273, ST147 ( 2/29 each ); ST1128, ST42, ST1031,ST764,ST629 and ST37(1/29 each).It is worth nothing that 4 new STs have been confirmed by the PubMlst database and named ST1369,ST1411,ST1412,ST1413.Among 2 ST groups, each ST group respectively included 2 STs ( ST395 and ST1369, ST147 and ST273 ) , the other 12 STs were single type, which ST11 ,ST15 and ST395 belongs to the CC258 clonal complex. Conclusion Gene polymorphism was existed on CRKPs of this region and the main types of 29 experimental strains were ST11 and ST15, and ST15 has been briefly local disseminated.