CAJ | 학술논문

目的：改良大鼠双侧颈总动脉永久性阻断模型（2VO）以适应急性全脑缺血研究。方法平行制作双侧颈总、颈内、颈外动脉永久性阻断（6VO）和2VO全脑缺血模型及假手术组，比较2种模型的死亡率、行为学评分、海马CA1区细胞坏死率、凋亡率及炎症因子的差异。结果6VO组24 h内死亡率（40％）与2VO组（0）、假手术组相比均明显增高，各个时间点行为学评分增高，神经元坏死、凋亡增加且炎症因子表达增高（均P＜0．05）。结论改良模型可加重大鼠脑损伤，适合急性全脑缺血研究。
목적：개량대서쌍측경총동맥영구성조단모형（2VO）이괄응급성전뇌결혈연구。방법평행제작쌍측경총、경내、경외동맥영구성조단（6VO）화2VO전뇌결혈모형급가수술조，비교2충모형적사망솔、행위학평분、해마CA1구세포배사솔、조망솔급염증인자적차이。결과6VO조24 h내사망솔（40％）여2VO조（0）、가수술조상비균명현증고，각개시간점행위학평분증고，신경원배사、조망증가차염증인자표체증고（균P＜0．05）。결론개량모형가가중대서뇌손상，괄합급성전뇌결혈연구。
Objective To adapt to the acute cerebral ischemia re-search , we modified the permanent bilateral common carotid artery occlu-sion (2VO) rat model.Methods Global ischemia models were estab-lished by permanent ligation of bilateral common, internal, external ca-rotid arteries (6VO) and the 2VO method respectively.The sham group were done simultaneously.Then the differences among three groups in mortality, behavioral score tests,pathological morphology, the expression of inflammatory factor by immunohistochemical method, and the percen-tage of cell apoptosis were compared.Results Twenty-four hours after operations, the mortality of 6VO group ( 40% ) was obviously higher than 2VO group ( 0 ) .The results of behavioral score, necrosis rate of neurons, the expression of inflammatory factors and cell apoptosis in hippocampus CA1 area in 6VO group were significantly higher than 2VO group and sham group ( P <0.05 ) .Conclusion The modified 6VO model is an ideal way to observe acute global ischemic injury in rats.