CAJ | 학술논문

以水稻糖质胚乳突变体 Sug-11与其野生型对照中花11为材料,通过对两者籽粒中可溶性总糖、蔗糖含量和淀粉含量以及有关淀粉品质理化指标的比较,结合籽粒灌浆过程中糖类物质含量、淀粉合成代谢关键酶活性和相关同工型基因转录表达水平的动态测定,从籽粒淀粉合成代谢角度,对水稻糖质突变体 Sug-11的籽粒糖类含量变化和千粒重下降的生理原因进行了分析。结果表明,Sug-11糖质突变体与其野生型在灌浆初期的可溶性糖和蔗糖含量差异并不明显,随着籽粒灌浆进程,两者间的籽粒糖分含量差异在灌浆中后期逐步趋于明显；与野生型相比,Sug-11糖质胚乳突变体的稻米直链淀粉含量和直链淀粉碘蓝值显著下降,而淀粉溶解度和支链淀粉碘蓝值则显著升高,糖质胚乳突变对稻米淀粉的理化特性也产生了明显的影响；在籽粒淀粉合成代谢的几个关键酶中,Sug-11糖质突变体籽粒中的 DBE 活性及其在灌浆过程中的动态变化与其野生型存在明显差异,揭示了胚乳糖质突变体 Sug-11籽粒中淀粉积累减少、糖分含量增加主要是由籽粒灌浆中后期的PUL 转录表达水平和 DBE 活性的大幅下降所引起的,而 Sug-11的籽粒灌浆不良和千粒重下降等现象,则与其 ADPGase活性在籽粒灌浆前期的显著下降存在一定的联系。
이수도당질배유돌변체 Sug-11여기야생형대조중화11위재료,통과대량자자립중가용성총당、자당함량화정분함량이급유관정분품질이화지표적비교,결합자립관장과정중당류물질함량、정분합성대사관건매활성화상관동공형기인전록표체수평적동태측정,종자립정분합성대사각도,대수도당질돌변체 Sug-11적자립당류함량변화화천립중하강적생리원인진행료분석。결과표명,Sug-11당질돌변체여기야생형재관장초기적가용성당화자당함량차이병불명현,수착자립관장진정,량자간적자립당분함량차이재관장중후기축보추우명현；여야생형상비,Sug-11당질배유돌변체적도미직련정분함량화직련정분전람치현저하강,이정분용해도화지련정분전람치칙현저승고,당질배유돌변대도미정분적이화특성야산생료명현적영향；재자립정분합성대사적궤개관건매중,Sug-11당질돌변체자립중적 DBE 활성급기재관장과정중적동태변화여기야생형존재명현차이,게시료배유당질돌변체 Sug-11자립중정분적루감소、당분함량증가주요시유자립관장중후기적PUL 전록표체수평화 DBE 활성적대폭하강소인기적,이 Sug-11적자립관장불량화천립중하강등현상,칙여기 ADPGase활성재자립관장전기적현저하강존재일정적련계。
With the rice sugary endosperm mutant (Sug-1 1 )and its wild-type (Zhonghua 1 1 )as materials,the total soluble sugar,sucrose contents,starch accumulation in filling grains and the starch physicochemical properties were compared to further reveal the temporal patterns of sugar content,several key enzymes activities and their isoform genes transcriptional expression level during the grain filling so as to analyze the physiological mechanism for Sug-1 1 whose sugars contents change and 1000-grain weight decline from the point of starch biosynthesis pathway in rice grains.The result showed that the differences in soluble sugar and sucrose contents between Sug-1 1 and its wild-type were not obvious at the initial stage of grain filling,but with the filling process it gradually became apparent.Secondly, the amylose(Am)content and BV(Am)of Sug-1 1 were significantly decreased while the starch solubility and BV (amylopectin,Ap)were increased compared with Zhonghua 1 1 ,indicating that the physicochemical properties of grain starch were significantly affected in the sugary endosperm mutant.Finally,among the key enzymes in starch synthetic metabolism,debranching enzyme(DBE),existed in Sug-1 1 seeds,were quite different from its wild type in activity and dynamic in the process of grain filling,and PUL transcriptional expression level and DBE activity strikingly decreased at the middle-late stage,resulting in the declined starch accumulation and increased sugar content in Sug-1 1 . However the poor filling and 1000-grain weight decline were,to some extent,associated with dropped ADPGase activity at the early grain filling stage.