CAJ | 학술논문

植物应对磷胁迫的方法之一是改变根系的构型.以水稻 SUMO 化 E3连接酶 SIZ1突变体 ossiz1为供试材料,研究了 OsSIZ1在水稻根发育中的作用以及其与磷胁迫、生长素之间的关系.与野生型相比,OsSIZ1抑制 ossiz1种子根和不定根的伸长,促进侧根密度的增加和根毛的增多.缺磷时,突变体 ossiz1的反应更强烈,即不定根伸长、侧根密度增大和根毛增多的趋势更加明显.说明 OsSIZ1参与调控水稻根构型的改变,低磷时效果更明显.ossiz1地上部和地下部的总磷浓度显著高于野生型,说明 OsSIZ1在水稻中负调控磷素的吸收利用.定量 RT-PCR 结果显示,ossiz1中 OsYUCCA1和 OsP IN1a/1b的相对表达量显著高于野生型,说明 OsSIZ1负调控根中生长素的合成与极性运输,并且缺磷时负调控作用减弱.结果表明,SUMO 化 E3连接酶 OsSIZ1调控缺磷条件下根构型的形成,而且这一过程可能是通过调控生长素分布完成的.
식물응대린협박적방법지일시개변근계적구형.이수도 SUMO 화 E3련접매 SIZ1돌변체 ossiz1위공시재료,연구료 OsSIZ1재수도근발육중적작용이급기여린협박、생장소지간적관계.여야생형상비,OsSIZ1억제 ossiz1충자근화불정근적신장,촉진측근밀도적증가화근모적증다.결린시,돌변체 ossiz1적반응경강렬,즉불정근신장、측근밀도증대화근모증다적추세경가명현.설명 OsSIZ1삼여조공수도근구형적개변,저린시효과경명현.ossiz1지상부화지하부적총린농도현저고우야생형,설명 OsSIZ1재수도중부조공린소적흡수이용.정량 RT-PCR 결과현시,ossiz1중 OsYUCCA1화 OsP IN1a/1b적상대표체량현저고우야생형,설명 OsSIZ1부조공근중생장소적합성여겁성운수,병차결린시부조공작용감약.결과표명,SUMO 화 E3련접매 OsSIZ1조공결린조건하근구형적형성,이차저일과정가능시통과조공생장소분포완성적.
Plants cope with the low P environment by altering their root architecture.Here,we studied the role of SUMO E3 ligase SIZ1 on the root development and architecture,and the relationship among OsSIZ1 ,phosphate stress and auxin using ossiz1 mutants.Compared with wild-type (WT),the ossiz1 mutation inhibited the elongation of seminal roots and adventitious roots,promoted lateral roots formation and root hair proliferation.However,the response of the ossiz1 mutation was stronger than WT under Pi deficiency,namely,the trend of root elongation and the increase of the lateral root density and the root hair number of ossiz1 were more obvious relative to WT seedlings. This suggests that OsSIZ1 regulates the root architecture,and the effect is more obvious under Pi deficiency.The total P concentration of shoots and roots in ossiz1 is higher than WT,this suggests that OsSIZ1 negatively regulates phosphorus acquisition and use.And relative expression of OsYUCCA1 and OsP I N1a/1b in ossiz1 roots is markedly up-regulated compared with WT.This suggests that OsSIZ1 negatively regulates the auxin biosynthesis and polar auxin transport,which is weakened under Pi-deficient conditions.Together,these results suggest that OsSIZ1 regulates Pi starvation-induced root architecture remodeling possibly through the control of auxin patterning.