CAJ | 학술논문

从中籼3037辐射突变体库中获得一个颖花开裂突变体(split glume1,sg 1),表型分析发现该突变体不仅颖壳异常,且伴随着生育期提前,植株变矮,颖花退化等现象,结实率也显著降低。遗传分析和分子生物学分析结果表明该突变受1对隐性核基因控制,并最终将其定位在水稻第1染色体上分子标记 M1-z21与 M1-z27之间,物理距离约为47 kb,这是一种控制颖花发育的新基因,通过预测发现该区段内存在6个开放阅读框。
종중선3037복사돌변체고중획득일개영화개렬돌변체(split glume1,sg 1),표형분석발현해돌변체불부영각이상,차반수착생육기제전,식주변왜,영화퇴화등현상,결실솔야현저강저。유전분석화분자생물학분석결과표명해돌변수1대은성핵기인공제,병최종장기정위재수도제1염색체상분자표기 M1-z21여 M1-z27지간,물리거리약위47 kb,저시일충공제영화발육적신기인,통과예측발현해구단내존재6개개방열독광。
A split glume mutant is from the mutant library of indica variety Zhongxian 3037 radiated by 60 Co γ-ray. Compared to its wild type,the mutant has not only split glumes,but also characterized by early heading,lower plant height,reduced seed setting rate and degenerated spikelets,which implies that the mutation has a pleiotropic effect. Genetic and molecular analysis indicated that the mutant was controlled by a recessive nuclear gene,temporally designated as split glume 1 (sg1 ),and then SG1 was fine mapped to a 47-kb DNA region between the STS markers M1-z21 and M1-z27 on 1 ,where six ORFs in total were predicted.On the basis of the location of SG1 ,it is deduced that SG1 is a novel gene regulating the rice glume development.