CAJ | 학술논문

目的：探讨新疆地区汉族鼻咽癌患者人类白细胞抗原（HLA）A2基因型表达差异与临床特征的关联性，比较 HLA-A*02阳性和阴性患者的近期疗效和远期疗效的差异。方法收集新疆医科大学附属肿瘤医院2007年1月－2014年1月接受全程 IMRT放疗的初治汉族鼻咽癌患者143例的外周血标本，采用聚合酶链反应／序列特异性引物的方法提取DNA样本，进行 HLA-A分型的检测，并将患者分为 HLA-A*02阳性组和阴性组，比较两组患者一般临床特征和近、远期疗效。结果143例汉族鼻咽癌患者共检出 HLA-A基因型13个，HLA-A*02表达患者94例，基因频率为38．7％，构成比为42．66％（122／286）。HLA-A*02阳性组和阴性组患者的年龄、性别、病理类型、TNM分期、近期疗效差异无统计学意义。两组患者的5年总生存率（OS）、无瘤生存率（DFS）及无远处转移生存率（DMFS）分别为84．43％和76．53％（χ2＝0．22，P ＝0．637）、82．79％和90．18％（χ2＝0．09，P ＝0．762）、71．23％和85．90％（χ2＝0．09，P ＝0．759），差异无统计学意义，HLA-A*02阳性组患者的5年生存率略低于阴性组患者。结论新疆汉族鼻咽癌的 HLA-A基因型中，HLA-A*02仍为优势基因。HLA-A*02基因型可能不能作为估计鼻咽癌患者预后的独立因素，需要联合其他因素进行综合分析。
목적：탐토신강지구한족비인암환자인류백세포항원（HLA）A2기인형표체차이여림상특정적관련성，비교 HLA-A*02양성화음성환자적근기료효화원기료효적차이。방법수집신강의과대학부속종류의원2007년1월－2014년1월접수전정 IMRT방료적초치한족비인암환자143례적외주혈표본，채용취합매련반응／서렬특이성인물적방법제취DNA양본，진행 HLA-A분형적검측，병장환자분위 HLA-A*02양성조화음성조，비교량조환자일반림상특정화근、원기료효。결과143례한족비인암환자공검출 HLA-A기인형13개，HLA-A*02표체환자94례，기인빈솔위38．7％，구성비위42．66％（122／286）。HLA-A*02양성조화음성조환자적년령、성별、병리류형、TNM분기、근기료효차이무통계학의의。량조환자적5년총생존솔（OS）、무류생존솔（DFS）급무원처전이생존솔（DMFS）분별위84．43％화76．53％（χ2＝0．22，P ＝0．637）、82．79％화90．18％（χ2＝0．09，P ＝0．762）、71．23％화85．90％（χ2＝0．09，P ＝0．759），차이무통계학의의，HLA-A*02양성조환자적5년생존솔략저우음성조환자。결론신강한족비인암적 HLA-A기인형중，HLA-A*02잉위우세기인。HLA-A*02기인형가능불능작위고계비인암환자예후적독립인소，수요연합기타인소진행종합분석。
Objective To analysis the relationship between the different human leukocyte antigen (HLA)A genotype and the clinical characteristics,in Han nasopharyngeal carcinoma(NPC)patients,also analyse the differences of the outcome between the HLA-A*02 and other genotype.Methods Collect One hundred and forty-three Han newly diagnosed NPC patients who received the whole IMRT at the Affiliated Tumor Hospital of Xinjiang Medical University between January 2007 and 2014.Blood samples were taken before treatment,polymerase chain reaction/sequence specific primers method was used to extract DNA,tested the HLA-A genotype, and divided the patients into 2 groups, HLA-A*02 positive and negative, compared the differences between the clinical characteristics of these two groups,compared the difference between the recent curative effect.Results In all 143 patients,13 HLA-A alleles had been tested,and a <br> total of 94 cases were detected 122 HLA-A*02 gene,the gene frequency was 38.7%,the proportion was 42.66% (122/286).Age,gender,pathological pattern,TNM stages and the short-termtheropeutic effect of the patients of HLA-A*02 positive and negative groups in the general clinical characteristics were seen no obvious distribution differences ,with the recent curative effect is also did not see obvious difference. Five years of two groups of patients with OS,DFS,DMFS,(Kaplan-Meier method was used to analyse two groups of patients 5-year overall survival (OS),disease-free survival (DFS),no distant metastasis and survival rate (DMFS),compared two groups of survival differences).Respectively,84.43% and 76.53%(χ2=0.22,P =0.637),82.79% and 90.18% (χ2=0.09,P =0.762),71.23% and 85.90% (χ2=0.09,P=0.759),those differences were no obvious difference,statistical analysis of HLA-A*02 positive patients survival rates was lower than negative one.Conclusion The Han nationality in Xinjiang HLA-A genotype of nasopharyngeal carcinoma,HLA-A*02 is still the dominant genes.HLA-A*02 genotype cannot esti-mate the prognosis of patients with nasopharyngeal carcinoma as independent factors,it need other factors comprehensive analysis.