新疆医科大学学报
新疆醫科大學學報
신강의과대학학보
JOURNAL OF XINJIANG MEDICAL UNIVERSITY
2015年
1期
6-10,16
,共6页
刘凯%吾甫尔·艾克木%王若峥
劉凱%吾甫爾·艾剋木%王若崢
류개%오보이·애극목%왕약쟁
鼻咽肿瘤%EB病毒%淋巴细胞亚群%流式细胞术
鼻嚥腫瘤%EB病毒%淋巴細胞亞群%流式細胞術
비인종류%EB병독%림파세포아군%류식세포술
nasopharyngeal cancer%EB virus%lymphocyte subsets%flow cytometry
目的：观察鼻咽癌患者放化疗前、后外周血淋巴细胞亚群的变化。方法应用流式细胞仪对40例健康正常人群（对照组）和32例鼻咽癌患者（鼻咽癌组）放化疗前、后的外周血T淋巴细胞亚群、B淋巴细胞及 NK细胞等进行检测，并比较鼻咽癌患者放疗前后和健康人群的检测值。结果鼻咽癌组放疗前外周血中 CD3＋、CD4＋、CD4＋／CD8＋、B细胞均值较对照组下降，差异均有统计学意义（t值分别为2．243、2．765、1．943、2．036，P 值分别为0．020、0．009、0．031、0．028）；外周血中 CD8＋、NK 细胞均值较对照组升高，差异均有统计学意义（t 值分别为－1．768、－2．865，P 值分别为0．043、0．007）；鼻咽癌组放疗后淋巴细胞亚群外周血中CD4＋、CD4＋／CD8＋均值较放疗前下降，差异均有统计学意义（t值分别为5．219、5．665，P 值分别为0．000、0．000）；外周血中 CD8＋、B细胞均值较放疗前升高，差异均有统计学意义（t值分别为－2．959、－2．922，P 值分别为0．004、0．004）；鼻咽癌组放疗前后CD3＋、NK细胞均值差异均无统计学意义（t值分别为1．576、－0．328，P 值分别为0．117、0．703）。结论鼻咽癌患者免疫功能下降，外周血T、B淋巴细胞及 NK细胞的检测对判断患者的免疫功能有参考作用。放疗可使鼻咽癌患者CD4＋／CD8＋明显下降，放疗期间应辅以免疫治疗。
目的：觀察鼻嚥癌患者放化療前、後外週血淋巴細胞亞群的變化。方法應用流式細胞儀對40例健康正常人群（對照組）和32例鼻嚥癌患者（鼻嚥癌組）放化療前、後的外週血T淋巴細胞亞群、B淋巴細胞及 NK細胞等進行檢測，併比較鼻嚥癌患者放療前後和健康人群的檢測值。結果鼻嚥癌組放療前外週血中 CD3＋、CD4＋、CD4＋／CD8＋、B細胞均值較對照組下降，差異均有統計學意義（t值分彆為2．243、2．765、1．943、2．036，P 值分彆為0．020、0．009、0．031、0．028）；外週血中 CD8＋、NK 細胞均值較對照組升高，差異均有統計學意義（t 值分彆為－1．768、－2．865，P 值分彆為0．043、0．007）；鼻嚥癌組放療後淋巴細胞亞群外週血中CD4＋、CD4＋／CD8＋均值較放療前下降，差異均有統計學意義（t值分彆為5．219、5．665，P 值分彆為0．000、0．000）；外週血中 CD8＋、B細胞均值較放療前升高，差異均有統計學意義（t值分彆為－2．959、－2．922，P 值分彆為0．004、0．004）；鼻嚥癌組放療前後CD3＋、NK細胞均值差異均無統計學意義（t值分彆為1．576、－0．328，P 值分彆為0．117、0．703）。結論鼻嚥癌患者免疫功能下降，外週血T、B淋巴細胞及 NK細胞的檢測對判斷患者的免疫功能有參攷作用。放療可使鼻嚥癌患者CD4＋／CD8＋明顯下降，放療期間應輔以免疫治療。
목적：관찰비인암환자방화료전、후외주혈림파세포아군적변화。방법응용류식세포의대40례건강정상인군（대조조）화32례비인암환자（비인암조）방화료전、후적외주혈T림파세포아군、B림파세포급 NK세포등진행검측，병비교비인암환자방료전후화건강인군적검측치。결과비인암조방료전외주혈중 CD3＋、CD4＋、CD4＋／CD8＋、B세포균치교대조조하강，차이균유통계학의의（t치분별위2．243、2．765、1．943、2．036，P 치분별위0．020、0．009、0．031、0．028）；외주혈중 CD8＋、NK 세포균치교대조조승고，차이균유통계학의의（t 치분별위－1．768、－2．865，P 치분별위0．043、0．007）；비인암조방료후림파세포아군외주혈중CD4＋、CD4＋／CD8＋균치교방료전하강，차이균유통계학의의（t치분별위5．219、5．665，P 치분별위0．000、0．000）；외주혈중 CD8＋、B세포균치교방료전승고，차이균유통계학의의（t치분별위－2．959、－2．922，P 치분별위0．004、0．004）；비인암조방료전후CD3＋、NK세포균치차이균무통계학의의（t치분별위1．576、－0．328，P 치분별위0．117、0．703）。결론비인암환자면역공능하강，외주혈T、B림파세포급 NK세포적검측대판단환자적면역공능유삼고작용。방료가사비인암환자CD4＋／CD8＋명현하강，방료기간응보이면역치료。
Objective To observe the changes of immune functions in patients with nasopharyngeal carcino-ma (NPC).To analyze the changes in cellular immune functions patients with NPC chemotherapy before and after chemoradiotherapy.Methods Flow cytometry was used to detect blood T lymphocyte subsets,B lymphocytes and NK cells of 40 cases of healthy people and 32 cases of patients with nasopharyngeal carci-noma before and after chemoradiotherapy.Comparisons were made in the testing results between patients with nasopharyngeal carcinoma before and after chemoradiotherapy and healthy people.Results CD3+, CD4+,CD4+/CD8+,B cells showed statistically significant differences between NPC patients before ra-diotherapy and healthy normal population control group,CD3+,CD4+,CD4+/CD8+,B cells had a downward trend (t values were 2.243,2.765,1.943,2.036,P values were 0.020,0.009,0.031,0.028) while CD8+,NK cells had an upward trend (t values were -1.768,-2.865,P values were 0.043, 0.007), which was statistically significant.CD4 +, CD4 +/CD8 + were significantly different in lymphocyte subsets levels of peripheral blood NPC patients before and after radiotherapy,CD4+,CD4+/ <br> CD8+ decreased gradually which had statistically significant difference (t values were 5.219,5.665,P val-ues were 0.000,0.000);CD8+,B cells rose gradually which had statistically significant difference (t val-ues were-2.959,-2.922,P values were 0.004,0.004).There was no statistically significant difference in CD3+,NK cells (t values were 1.576,-0.328,P values were 0.117,0.703).Conclusion NPC patients show a low immune function,levels of Peripheral blood B and T lymphocyte subsets and NK cells are good references in j udging patient's immune function.Radiotherapy significantly decreases the percentages of CD4+/CD8.Therefore,radiotherapy should be supplemented by immunotherapy.
