CAJ | 학술논문

目的：建立榆叶合叶子黄酮类成分的微乳薄层色谱鉴别方法。方法以十二烷基硫酸钠（SDS）-正丁醇-正庚烷-水组成的微乳体系为展开剂，对榆叶合叶子的全草和花的提取物进行薄层层析，考察微乳薄层中的微乳液类型、酸度等因素对黄酮类物质分离效果的影响。结果含水量75％（O／W）的微乳液-甲酸（9∶1）为适宜展开剂，与传统有机溶剂展开剂相比，分离得到的斑点数目更多，更加清晰，分离度更好。结论黄酮类成分的微乳薄层鉴别方法简便易行，重复性好，可作为榆叶合叶子药材的薄层鉴别方法。
목적：건립유협합협자황동류성분적미유박층색보감별방법。방법이십이완기류산납（SDS）-정정순-정경완-수조성적미유체계위전개제，대유협합협자적전초화화적제취물진행박층층석，고찰미유박층중적미유액류형、산도등인소대황동류물질분리효과적영향。결과함수량75％（O／W）적미유액-갑산（9∶1）위괄의전개제，여전통유궤용제전개제상비，분리득도적반점수목경다，경가청석，분리도경호。결론황동류성분적미유박층감별방법간편역행，중복성호，가작위유협합협자약재적박층감별방법。
Objective To establish a new method on the identification of flavone in Filipendula ulmari by microemulsion thin-layer chromatography.Methods The flowers and herbals samples of Filipendula ulmari were eluted with the developer of SDS-n-C4 H9 OH-n-C7 H16-H2 O to investigate the effects of the microemulsion types and acidity.Results The flavone was separated entirely by the developer of water content of 75% (O/W)of microemulsion.Compared with the other organic developers,more spots were i-dentified and the resolution was improved markedly.Conclusion This method of microemulsion thin-layer chromatography was easily adopted, with good repeatability, which could be used as a quality identification of flavone in Filipendula ulmari .