CAJ | 학술논문

目的：研究苦豆子总生物碱对小鼠乳腺癌4T1细胞体外增殖抑制及诱导凋亡作用。方法苦豆子总生物碱提取并纯化，处理成澄清的药物溶液后用于细胞给药。CCK-8法测定不同浓度的苦豆子总生物碱作用24 h后4T1细胞存活率。流式细胞术检测给药24 h后4T1细胞凋亡率。结果与对照组比较，实验组药物浓度为5．00 mg／mL时，4T1细胞存活率为63．05％；药物浓度为10．00 mg／mL时，4T1细胞存活率为20．30％，随着给药浓度的增加，细胞存活率呈明显下降趋势。实验组药物浓度为6．25 mg／mL时，细胞凋亡率为11．4％；药物浓度为10．00 mg／mL时，细胞凋亡率为41．7％，随着给药浓度的增加，细胞凋亡率明显增加。结论苦豆子总生物碱能诱导细胞凋亡，对小鼠乳腺癌4T1细胞的增殖有明显抑制作用。
목적：연구고두자총생물감대소서유선암4T1세포체외증식억제급유도조망작용。방법고두자총생물감제취병순화，처리성징청적약물용액후용우세포급약。CCK-8법측정불동농도적고두자총생물감작용24 h후4T1세포존활솔。류식세포술검측급약24 h후4T1세포조망솔。결과여대조조비교，실험조약물농도위5．00 mg／mL시，4T1세포존활솔위63．05％；약물농도위10．00 mg／mL시，4T1세포존활솔위20．30％，수착급약농도적증가，세포존활솔정명현하강추세。실험조약물농도위6．25 mg／mL시，세포조망솔위11．4％；약물농도위10．00 mg／mL시，세포조망솔위41．7％，수착급약농도적증가，세포조망솔명현증가。결론고두자총생물감능유도세포조망，대소서유선암4T1세포적증식유명현억제작용。
Objective To explore the proliferation inhibition and apoptosis effect of the total alkaloid of so-phora alopecuroides (TASA)on mouse mammary tumor 4T1 in vitro.Methods TASA was extracted and purified,and then TASA was administrated to mouse mammary tumor 4T1 cells.The inhibitory effect of TASA at different concentrations on 4T1 cells was determinated by CCK-8 assay at the time point of 24 h. The effect of TASA on 4T1 cells apoptosis was detected by flow cytometry.Results With the drug con-centration of 5.00 mg/mL,4T1 cells survival rate was 63.05%,while the drug concentration reached to 10.00 mg/mL,4T1 cells survival rate was 20.30%,which suggested that 4T1 cells survival rate signifi-cantly declined with the increase of drug concentration.With the drug concentration of 6 .2 5 mg/mL,the apoptosis rate was 11.4%,while the drug concentration was 10.00 mg/mL,the apoptosis rate was 41.7%, which showed that apoptosis rate was increased significantly with the drug level increasing.Conclusion <br> TASA could induce cell apoptosis of 4T1 cells,and it had a remarkable inhibitory effect on the prolifera-tion of 4T1 cells.