CAJ | 학술논문

目的：研究破布木果提取物对小鼠脾淋巴细胞的增殖作用。方法不同浓度的破布木果提取物对刀豆蛋白 A（concanavalin A，ConA）、脂多糖试剂（LPS）体外处理的小鼠脾淋巴细胞的作用；采用 MTT法测定淋巴细胞的增殖能力。结果破布木果提取液在2．69～86 mg／mL范围内，均与 ConA、LPS诱导的小鼠脾淋巴细胞的增殖在体外具有协同作用；破布木果多糖在21．50～86 mg／mL范围内，与 ConA诱导的小鼠脾淋巴细胞的增殖在体外具有协同作用；在10．75～86 mg／mL范围内，与 LPS诱导的小鼠脾淋巴细胞的增殖在体外具有协同作用；迷迭香酸标准品作为药物的阳性对照，均与 ConA、LPS诱导的小鼠脾淋巴细胞的增殖在体外具有协同作用。结论初步明确破布木果提取液、破布木果多糖、迷迭香酸标准品具有一定的免疫调节作用。
목적：연구파포목과제취물대소서비림파세포적증식작용。방법불동농도적파포목과제취물대도두단백 A（concanavalin A，ConA）、지다당시제（LPS）체외처리적소서비림파세포적작용；채용 MTT법측정림파세포적증식능력。결과파포목과제취액재2．69～86 mg／mL범위내，균여 ConA、LPS유도적소서비림파세포적증식재체외구유협동작용；파포목과다당재21．50～86 mg／mL범위내，여 ConA유도적소서비림파세포적증식재체외구유협동작용；재10．75～86 mg／mL범위내，여 LPS유도적소서비림파세포적증식재체외구유협동작용；미질향산표준품작위약물적양성대조，균여 ConA、LPS유도적소서비림파세포적증식재체외구유협동작용。결론초보명학파포목과제취액、파포목과다당、미질향산표준품구유일정적면역조절작용。
Objective To investigate the proliferative effects of extracts from Cordia dichotoma seeds on spleen lymphocytes of mice.Methods Detect the effects of the extracts with different concentrations from Cordia dichotoma seeds on mouse spleen lymphocytes by concanavalin A (ConA)and lipopolysaccharide (LPS)in vitro treatment;Detect the proliferation ability of lymphocyte by MTT assay.Results The ex-tracts of Cordia dichotoma seeds in the range of 2.69-86 mg/mL had a synergistic effect in vitro on the mouse spleen lymphocytes proliferation induced by ConA and LPS;polysaccharides of Cordia dichotoma seeds in the range of 21.50-86 mg/mL had synergistic effects in vitro on the spleen lymphocyte prolifera-tion induced by ConA;polysaccharides in the range of 10.75-86 mg/mL had synergistic effects in vitro on the spleen lymphocyte proliferation induced by LPS;rosmarinic acid standard,used as the positive control drug,had a synergistic effect in vitro on the mouse spleen lymphocytes proliferation induced by ConA and LPS.Conclusion This primary study showed that the extracts from Cordia dichotoma seeds,polysaccha-rides of Cordia dichotoma seeds and rosmarinic acid standards had some immunomodulatory effects.