CAJ | 학술논문

为探讨羊经布鲁氏菌病疫苗免疫后的血清抗体消长规律，本研究分别对绵羊和山羊进行布鲁氏菌S2株疫苗不同剂量的灌服，于免疫前和免疫后不同时间采集血清样品，采用虎红平板凝集试验和试管凝集试验进行血清抗体检测。结果表明，绵羊及山羊均在免疫后20d时血清抗体阳性率达到最高峰，之后呈下降趋势，免疫后90天降至低点。免疫后90至360d，绵羊组抗体阳性率几乎一直维持在14%以下，各时段无显著性差异（P>0.05）；山羊组抗体阳性率在免疫后90至360d期间时高时低，起伏较大，无明显规律。试验期间，山羊的抗体阳性率普遍高于绵羊，且降升幅度较大。同时，研究表明，同种羊100亿和200亿两个免疫剂量组的抗体阳性率差异不大。
위탐토양경포로씨균병역묘면역후적혈청항체소장규률，본연구분별대면양화산양진행포로씨균S2주역묘불동제량적관복，우면역전화면역후불동시간채집혈청양품，채용호홍평판응집시험화시관응집시험진행혈청항체검측。결과표명，면양급산양균재면역후20d시혈청항체양성솔체도최고봉，지후정하강추세，면역후90천강지저점。면역후90지360d，면양조항체양성솔궤호일직유지재14%이하，각시단무현저성차이（P>0.05）；산양조항체양성솔재면역후90지360d기간시고시저，기복교대，무명현규률。시험기간，산양적항체양성솔보편고우면양，차강승폭도교대。동시，연구표명，동충양100억화200억량개면역제량조적항체양성솔차이불대。
To investigate the pattern of increasing or decreasing of serum antibody in sheep and goats immunized with brucellosis vaccine,RPST and tube agglutination test were used in the study to determine the level of serum antibody in sheep and goats before and after immunization with Brucella strain S2 vaccine. The results indicated that the serum antibody positive rate in sheep reached the highest level 20 DPI,then started to decrease and remained relatively low level 90-360 DPI,mostly below 6%,and relatively higher in few individuals,up to 14%. The positive rate in goats reached the highest 20 DPI,then decreased and remained low or relatively low level 90-360 DPI,but with decreasing or increasing sometimes. Comparatively,antibody positive rate in goats was generally higher than in sheep,and the descending and ascending range was bigger. It was also shown that there was no significant difference in antibody posi-tive rate between 10 billion CFU dose group and 20 billion CFU dose group in same species.