CAJ | 학술논문

川明参多糖及其硫酸化物对免疫低下小鼠的影响
천명삼다당급기류산화물대면역저하소서적영향
Effect of Chuanmingshen violaceum polysaccharides and its sulfated derivatives on immunosuppression induced by cyclophosphamide in mice

万方数据

中国免疫学杂志中國免疫學雜誌 중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2015年 1期 52-55,60 ,共5页

赵兴洪%殷中琼%贾仁勇%赵兴芳%宋旭%徐娇%李莉%戴书俊%康帅%李正文%彭练慈%陈振振%胡志强

조흥홍%은중경%가인용%조흥방%송욱%서교%리리%대서준%강수%리정문%팽련자%진진진%호지강

川明参多糖%硫酸化川明参多糖%免疫低下%免疫器官指数%脾脏淋巴细胞增殖%细胞因子川明參多糖%硫痠化川明參多糖%免疫低下%免疫器官指數%脾髒淋巴細胞增殖%細胞因子
천명삼다당%류산화천명삼다당%면역저하%면역기관지수%비장림파세포증식%세포인자
Chuanmingshen violaceum polysaccharides%Solfated Chuanmingshen violaceum polysaccharides%Immuno-suppression%Immune organs indexes%Spleen lymphocytes proliferation%Cytokines

目的：研究川明参多糖（ Chuanmingshen violaceum polysaccharides， CVP ）及硫酸化川明参多糖（ Solfated chuanmingshen violaceum polysaccharides，SCVP）对免疫低下小鼠部分免疫指标的影响。方法：采用环磷酰胺来诱导小鼠免疫低下；胸腺指数和脾脏指数来衡量免疫器官变化；MTT法检测CVP及SCVP不同剂量对静息期脾脏淋巴细胞、伴刀豆球蛋白A （ ConA）处理的脾脏淋巴细胞、脂多糖（ LPS）处理的脾脏淋巴细胞增殖活性的影响；血清IFN－γ和IL－2采用ELISA试剂盒检测。结果：CVP和SCVP可以通过提高免疫低下小鼠免疫器官指数、各处理脾脏淋巴细胞增殖活性和血清IFN－γ和IL－2含量，从而提高机体的免疫力。结论：CVP和SCVP可作为免疫增强剂开发应用于临床。
목적：연구천명삼다당（ Chuanmingshen violaceum polysaccharides， CVP ）급류산화천명삼다당（ Solfated chuanmingshen violaceum polysaccharides，SCVP）대면역저하소서부분면역지표적영향。방법：채용배린선알래유도소서면역저하；흉선지수화비장지수래형량면역기관변화；MTT법검측CVP급SCVP불동제량대정식기비장림파세포、반도두구단백A （ ConA）처리적비장림파세포、지다당（ LPS）처리적비장림파세포증식활성적영향；혈청IFN－γ화IL－2채용ELISA시제합검측。결과：CVP화SCVP가이통과제고면역저하소서면역기관지수、각처리비장림파세포증식활성화혈청IFN－γ화IL－2함량，종이제고궤체적면역력。결론：CVP화SCVP가작위면역증강제개발응용우림상。
Objective:In oder to investigate the effect of Chuanmingshen violaceum polysaccharides ( CVP) and Solfated Chua-nmingshen violaceum polysaccharides ( SCVP) on immunosuppression induced by cyclophosphamide ( CY) in mice.Methods: CY were used to induce immunosuppression in mice;Spleen and thymus indexes were used to evaluate the immune organs indexes;the [3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltet-razolium bromide,MTT] method was used to detect the proliferation of spleen lymphocytes of each group;the concentrations of IFN-γand IL-2 were assayed by ELISA kit.Results: SCVP and CVP could resist immunosuppression by promoting lymphocyte proliferation, increasing the contents of IFN-γ and IL-2, promoting immune organs development in immunosuppressive mice induced by CY.Conclusion:SCVP and CVP exhibited the potential to used as immunopotentiator.