CAJ | 학술논문

目的：初步探讨B7阻断剂CD28与细胞毒T淋巴细胞相关抗原4免疫球蛋白（CTLA－4Ig）对B6．MRL－Faslpr／J狼疮小鼠脾脏Th17和调节性T细胞（ Treg细胞）表达的影响与其对小鼠狼疮样病征干预作用之间的相关性。方法：将4个月龄大小雌性B6．MRL－Faslpr／J狼疮小鼠16只，随机分为观察组（Ⅰ组）和对照组（Ⅱ组），分别静脉注射CTLA－4Ig及等量PBS，检测小鼠干预前后24 h尿蛋白、ANA抗体、ds－DNA抗体及干预结束2周后血清IL－17A、脾脏中Th17细胞和Treg细胞百分比。结果：末次干预2周后Ⅰ组的24 h尿蛋白、血清ANA及ds－DNA较Ⅱ组下降均有统计学意义（均P＜0．05）。末次干预2周后Ⅰ组血清中IL－17A、脾脏Th17细胞比例均较Ⅱ组低，而脾脏的Treg细胞占CD4＋T淋巴细胞的比例高于Ⅱ组，差异均有统计学意义（均P＜0．05）。结论：CTLA4－Ig具有减轻B6．MRL－Faslpr／J狼疮鼠狼疮样病征的作用；上调Treg细胞、下调Th17细胞可能是CTLA－4Ig减轻B6．MRL－Faslpr／J狼疮鼠狼疮样病征的重要机制之一。
목적：초보탐토B7조단제CD28여세포독T림파세포상관항원4면역구단백（CTLA－4Ig）대B6．MRL－Faslpr／J랑창소서비장Th17화조절성T세포（ Treg세포）표체적영향여기대소서랑창양병정간예작용지간적상관성。방법：장4개월령대소자성B6．MRL－Faslpr／J랑창소서16지，수궤분위관찰조（Ⅰ조）화대조조（Ⅱ조），분별정맥주사CTLA－4Ig급등량PBS，검측소서간예전후24 h뇨단백、ANA항체、ds－DNA항체급간예결속2주후혈청IL－17A、비장중Th17세포화Treg세포백분비。결과：말차간예2주후Ⅰ조적24 h뇨단백、혈청ANA급ds－DNA교Ⅱ조하강균유통계학의의（균P＜0．05）。말차간예2주후Ⅰ조혈청중IL－17A、비장Th17세포비례균교Ⅱ조저，이비장적Treg세포점CD4＋T림파세포적비례고우Ⅱ조，차이균유통계학의의（균P＜0．05）。결론：CTLA4－Ig구유감경B6．MRL－Faslpr／J랑창서랑창양병정적작용；상조Treg세포、하조Th17세포가능시CTLA－4Ig감경B6．MRL－Faslpr／J랑창서랑창양병정적중요궤제지일。
Objective:To observe the correlation between the B7 blocker,CD28 and cytotoxic T lymphocyte-associated antigen 4 immunoglobulin (CTLA-4Ig) impacting on Th17 and Treg cells expressed in the spleen of lupus mice and its intervention role in lupus-like symptoms of B6.MRL-Faslpr/J lupus mice.Methods:Sixteen 4-month-old female B6.MRL-Faslpr/J mice were selected and randomly divided into treatment group ( groupⅠ) and control group ( groupⅡ);injected the same amount of CTLA-4Ig and PBS intra-venously,checked their 24 hour urine protein ,ANA antibody,ds-DNA antibodies before and after the intervention.Two weeks after the intervention ,detected serum IL-17A,and the percentage of Th17 and Treg cells in their spleen.Results:Two weeks after the last inter-vention,24-hour urine protein,serum ANA and ds-DNA in groupⅠdecreased,and all the differences were statistically significant (P<0.05) compared with groupⅡ.Two weeks after the last intervention,serum IL-17A and the proportion of Th17 cells in the spleen in groupⅠwere lower than those in groupⅡ, but Treg cells in CD4+T lymphocytes was higher than that in groupⅡ,all the differences were statistically significant ( P<0.05).Conclusion:CTLA4-Ig can relieve the lupus-like symptoms in lupus mice;raising the number of Treg cells and decreasing the number of Th17 cells may be one of the important mechanisms for CTLA-4Ig to alleviate lupus-like symptoms in B6.MRL-Faslpr/J mice.