CAJ | 학술논문

目的：通过检测中性粒细胞激活素受体表达以及细胞活性，阐述激活素A调控中性粒细胞的作用。方法：分离小鼠腹腔中性粒细胞；通过免疫荧光细胞化学染色及流式细胞术检测中性粒细胞激活素ⅡA型受体（ ActRⅡA）表达；激活素A刺激后，Western blot检测中性粒细胞Smad3表达；检测呼吸爆发、NO分泌及吞噬能力的变化以确定中性粒细胞活性。结果：分离的小鼠腹腔中性粒细胞纯度大于90％；免疫荧光细胞化学染色显示中性粒细胞可以表达ActRⅡA，流式细胞术检测结果显示Gr－1与ActRⅡA双阳性细胞约41．1％；激活素A刺激后，中性粒细胞p－Smad3表达上调，ROS及O2－产生升高（ P＜0．05），NO分泌增加（P＜0．01），流式细胞术检测结果显示激活素A还可以明显促进中性粒细胞对荧光微球的吞噬（P＜0．01）。结论：中性粒细胞可以表达激活素受体及其信号传导蛋白，激活素A对中性粒细胞活化及功能具有重要的调节作用。
목적：통과검측중성립세포격활소수체표체이급세포활성，천술격활소A조공중성립세포적작용。방법：분리소서복강중성립세포；통과면역형광세포화학염색급류식세포술검측중성립세포격활소ⅡA형수체（ ActRⅡA）표체；격활소A자격후，Western blot검측중성립세포Smad3표체；검측호흡폭발、NO분비급탄서능력적변화이학정중성립세포활성。결과：분리적소서복강중성립세포순도대우90％；면역형광세포화학염색현시중성립세포가이표체ActRⅡA，류식세포술검측결과현시Gr－1여ActRⅡA쌍양성세포약41．1％；격활소A자격후，중성립세포p－Smad3표체상조，ROS급O2－산생승고（ P＜0．05），NO분비증가（P＜0．01），류식세포술검측결과현시격활소A환가이명현촉진중성립세포대형광미구적탄서（P＜0．01）。결론：중성립세포가이표체격활소수체급기신호전도단백，격활소A대중성립세포활화급공능구유중요적조절작용。
Objective:To study the role of activin A in regulation of neutrophil function by detecting activin receptor expression and cellular activities.Methods:Peritoneal neutrophils were isolated in mouse.After the neutrophils were stimulated with activin A,the expression of ActRⅡA on neutrophils was examined by immunofluorescence and flow cytometry.Expression of Smad3 in neutrophils was analyzed by Western blot.Assays of neutrophils function were performed by detecting respiratory burst, production of NO and phagocytosis.Results:The isolated cells were composed of more than 90% peritoneal neutrophils.ActRⅡA was expressed on mouse neutrophils and Gr-1/ActRⅡA double-positive cells were 41.1%.Activin A promoted Smad3 phosphorylation in neutrophils,increased the production of ROS and O2-(P<0.05),enhanced secretion of NO and phagocytosis of mouse neutrophils(P<0.01),and promoted fluorescent microsphere phagocytosis of neutrophils by flow cytometry ( P<0.01 ) .Conclusion: Activin receptor and activin signaling protein were expressed on mouse neutrophils,activin A might play an important regulatory role in activation and function of neutrophils.