CAJ | 학술논문

目的：分析H7N9流感病毒的HA、NA蛋白的抗原表位；预测H7N9流感病毒相关的HLA－Ⅱ类等位基因及易感人群。方法：软件分析HA、NA的同源性、B细胞表位、T细胞表位以及与HA、NA结合力强的HLA－Ⅱ类等位基因；并根据该等位基因在亚洲不同地域的基因频率，预测H7N9的易感人群。结果：H7N9流感病毒株之间氨基酸序列相对保守；HA具有10个B细胞表位和15个T细胞表位；NA有12个B细胞表位和9个T细胞表位；HLA等位基因DRB1*0701与HA、NA具有强结合力，在新疆、哈尔滨、山东、辽宁、北京、石家庄、天津等多个中国北方地区人群中的基因频率较高，高于广东、云南、台湾地区、日本和韩国。结论：预测了HA、NA的抗原性表位，为H7 N9流感病毒的疫苗研究提供了理论基础；HLA－DRB1*0701与H7 N9流感病毒高度相关；H7 N9流感病毒在新疆、哈尔滨、山东、辽宁、北京、石家庄、天津地区更易传播。
목적：분석H7N9류감병독적HA、NA단백적항원표위；예측H7N9류감병독상관적HLA－Ⅱ류등위기인급역감인군。방법：연건분석HA、NA적동원성、B세포표위、T세포표위이급여HA、NA결합력강적HLA－Ⅱ류등위기인；병근거해등위기인재아주불동지역적기인빈솔，예측H7N9적역감인군。결과：H7N9류감병독주지간안기산서렬상대보수；HA구유10개B세포표위화15개T세포표위；NA유12개B세포표위화9개T세포표위；HLA등위기인DRB1*0701여HA、NA구유강결합력，재신강、합이빈、산동、료녕、북경、석가장、천진등다개중국북방지구인군중적기인빈솔교고，고우엄동、운남、태만지구、일본화한국。결론：예측료HA、NA적항원성표위，위H7 N9류감병독적역묘연구제공료이론기출；HLA－DRB1*0701여H7 N9류감병독고도상관；H7 N9류감병독재신강、합이빈、산동、료녕、북경、석가장、천진지구경역전파。
Objective:To compare the amino acid sequences difference of HA,NA novel influenza virus A/H7N9 isolates, decipher possible B cell epitopes and T cell epitopes of HA,NA protein,and analyze the association between susceptibility and HLA polymorphisms.Methods:The amino acid sequences of novel influenza A ( H7N9) virus were downloaded from Genbank.Phylogenetic trees were constructed based on the amino acid sequences of HA and NA by using software Clustal X and MEGA 4.0.B cell and T cell epitopes were respectively predicted with Protean software and NetMHCⅡ2.2 Server online server.Results:The homology of HA and NA proteins of H7N9 virus was high.10 B cell epitopes and 15 T cell epitopes were randomly distributed throughout HA sequence and 12 B cell epitopes and 9 T cell epitopes were randomly distributed throughout NA sequence.HLA-DRB1*0701 allele which was commonly observed in Northern Chinese population have a high binding affinity for 9-mer peptides of HA and NA proteins.Conclusion:The prediction of B and T cell epitopes of HA and NA proteins with multiple methods benefits the research and development of vaccine against human infection with avian influenza A H7N9 virus.HLA-DRB1*0701 allele may contribute to susceptibility to novel influenza A (H7N9) virus.H7N9 influenza virus is more easily spread in Urumqi,Harbin,Shandong Province,Liaoning Province,Beijing, Shijiazhuang and Tianjin of China.